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Fluorescence microscopy

Overview of attention for article published in Nature Methods, November 2005
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (98th percentile)
  • High Attention Score compared to outputs of the same age and source (93rd percentile)

Mentioned by

news
3 news outlets
blogs
3 blogs
twitter
1 X user
patent
42 patents
wikipedia
3 Wikipedia pages
googleplus
1 Google+ user

Citations

dimensions_citation
1315 Dimensions

Readers on

mendeley
3608 Mendeley
citeulike
13 CiteULike
connotea
13 Connotea
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Title
Fluorescence microscopy
Published in
Nature Methods, November 2005
DOI 10.1038/nmeth817
Pubmed ID
Authors

Jeff W Lichtman, José-Angel Conchello

Abstract

Although fluorescence microscopy permeates all of cell and molecular biology, most biologists have little experience with the underlying photophysical phenomena. Understanding the principles underlying fluorescence microscopy is useful when attempting to solve imaging problems. Additionally, fluorescence microscopy is in a state of rapid evolution, with new techniques, probes and equipment appearing almost daily. Familiarity with fluorescence is a prerequisite for taking advantage of many of these developments. This review attempts to provide a framework for understanding excitation of and emission by fluorophores, the way fluorescence microscopes work, and some of the ways fluorescence can be optimized.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3,608 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 44 1%
United Kingdom 22 <1%
Germany 22 <1%
France 10 <1%
Netherlands 10 <1%
Spain 9 <1%
Mexico 7 <1%
Switzerland 6 <1%
India 6 <1%
Other 66 2%
Unknown 3406 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 824 23%
Student > Bachelor 510 14%
Student > Master 501 14%
Researcher 465 13%
Student > Doctoral Student 161 4%
Other 365 10%
Unknown 782 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 812 23%
Biochemistry, Genetics and Molecular Biology 410 11%
Chemistry 392 11%
Physics and Astronomy 317 9%
Engineering 307 9%
Other 531 15%
Unknown 839 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 52. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 February 2024.
All research outputs
#821,969
of 26,017,215 outputs
Outputs from Nature Methods
#1,068
of 5,404 outputs
Outputs of similar age
#1,626
of 162,539 outputs
Outputs of similar age from Nature Methods
#2
of 31 outputs
Altmetric has tracked 26,017,215 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,404 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 36.7. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 162,539 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 31 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.