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Sequence context and crosslinking mechanism affect the efficiency of in vivo capture of a protein–protein interaction

Overview of attention for article published in Biospectroscopy, January 2014
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Title
Sequence context and crosslinking mechanism affect the efficiency of in vivo capture of a protein–protein interaction
Published in
Biospectroscopy, January 2014
DOI 10.1002/bip.22395
Pubmed ID
Authors

Jody K. Lancia, Adaora Nwokoye, Amanda Dugan, Cassandra Joiner, Rachel Pricer, Anna K. Mapp

Abstract

Protein-protein interactions (PPIs) are essential for implementing cellular processes and thus methods for the discovery and study of PPIs are highly desirable. An emerging method for capturing PPIs in their native cellular environment is in vivo covalent chemical capture, a method that uses nonsense suppression to site specifically incorporate photoactivable unnatural amino acids (UAAs) in living cells. However, in one study we found that this method did not capture a PPI for which there was abundant functional evidence, a complex formed between the transcriptional activator Gal4 and its repressor protein Gal80. Here we describe the factors that influence the success of covalent chemical capture and show that the innate reactivity of the two UAAs utilized, (p-benzoylphenylalanine (pBpa) and p-azidophenylalanine (pAzpa)), plays a profound role in the capture of Gal80 by Gal4. Based upon these data, guidelines are outlined for the successful use of in vivo photo-crosslinking to capture novel PPIs and to characterize the interfaces. © 2013 Wiley Periodicals, Inc. Biopolymers 101: 391-397, 2014.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 31 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 15 48%
Professor 4 13%
Researcher 3 10%
Student > Doctoral Student 3 10%
Student > Bachelor 1 3%
Other 2 6%
Unknown 3 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 29%
Agricultural and Biological Sciences 9 29%
Chemistry 6 19%
Business, Management and Accounting 1 3%
Immunology and Microbiology 1 3%
Other 1 3%
Unknown 4 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 April 2016.
All research outputs
#16,722,190
of 25,374,917 outputs
Outputs from Biospectroscopy
#1,541
of 1,867 outputs
Outputs of similar age
#195,527
of 320,698 outputs
Outputs of similar age from Biospectroscopy
#9
of 17 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,867 research outputs from this source. They receive a mean Attention Score of 3.8. This one is in the 16th percentile – i.e., 16% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 320,698 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.