Title |
Transcription Factors Modulate c-Fos Transcriptional Bursts
|
---|---|
Published in |
Cell Reports, June 2014
|
DOI | 10.1016/j.celrep.2014.05.053 |
Pubmed ID | |
Authors |
Adrien Senecal, Brian Munsky, Florence Proux, Nathalie Ly, Floriane E. Braye, Christophe Zimmer, Florian Mueller, Xavier Darzacq |
Abstract |
Transcription is a stochastic process occurring mostly in episodic bursts. Although the local chromatin environment is known to influence the bursting behavior on long timescales, the impact of transcription factors (TFs)--especially in rapidly inducible systems--is largely unknown. Using fluorescence in situ hybridization and computational models, we quantified the transcriptional activity of the proto-oncogene c-Fos with single mRNA accuracy at individual endogenous alleles. We showed that, during MAPK induction, the TF concentration modulates the burst frequency of c-Fos, whereas other bursting parameters remain mostly unchanged. By using synthetic TFs with TALE DNA-binding domains, we systematically altered different aspects of these bursts. Specifically, we linked the polymerase initiation frequency to the strength of the transactivation domain and the burst duration to the TF lifetime on the promoter. Our results show how TFs and promoter binding domains collectively act to regulate different bursting parameters, offering a vast, evolutionarily tunable regulatory range for individual genes. |
X Demographics
Geographical breakdown
Country | Count | As % |
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France | 2 | 50% |
Japan | 1 | 25% |
Unknown | 1 | 25% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 75% |
Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 3 | 1% |
United States | 3 | 1% |
United Kingdom | 2 | <1% |
Portugal | 1 | <1% |
Italy | 1 | <1% |
Switzerland | 1 | <1% |
Netherlands | 1 | <1% |
Luxembourg | 1 | <1% |
Unknown | 251 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 68 | 26% |
Researcher | 58 | 22% |
Student > Bachelor | 27 | 10% |
Student > Master | 21 | 8% |
Student > Doctoral Student | 14 | 5% |
Other | 39 | 15% |
Unknown | 37 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 84 | 32% |
Agricultural and Biological Sciences | 77 | 29% |
Physics and Astronomy | 19 | 7% |
Medicine and Dentistry | 11 | 4% |
Engineering | 10 | 4% |
Other | 18 | 7% |
Unknown | 45 | 17% |