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Quantitative Proteome Profiling of CNS-Infiltrating Autoreactive CD4+ Cells Reveals Selective Changes during Experimental Autoimmune Encephalomyelitis

Overview of attention for article published in Journal of Proteome Research, July 2014
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Title
Quantitative Proteome Profiling of CNS-Infiltrating Autoreactive CD4+ Cells Reveals Selective Changes during Experimental Autoimmune Encephalomyelitis
Published in
Journal of Proteome Research, July 2014
DOI 10.1021/pr500158r
Pubmed ID
Authors

Michelle E. Turvey, Tomas Koudelka, Iain Comerford, Judith M. Greer, William Carroll, Claude C. A. Bernard, Peter Hoffmann, Shaun R. McColl

Abstract

Experimental autoimmune encephalomyelitis (EAE) is a murine model of multiple sclerosis, a chronic neurodegenerative and inflammatory autoimmune condition of the central nervous system (CNS). Pathology is driven by the infiltration of autoreactive CD4(+) lymphocytes into the CNS, where they attack neuronal sheaths causing ascending paralysis. We used an isotope-coded protein labeling approach to investigate the proteome of CD4(+) cells isolated from the spinal cord and brain of mice at various stages of EAE progression in two EAE disease models: PLP139-151-induced relapsing-remitting EAE and MOG35-55-induced chronic EAE, which emulate the two forms of human multiple sclerosis. A total of 1120 proteins were quantified across disease onset, peak-disease, and remission phases of disease, and of these 13 up-regulated proteins of interest were identified with functions relating to the regulation of inflammation, leukocyte adhesion and migration, tissue repair, and the regulation of transcription/translation. Proteins implicated in processes such as inflammation (S100A4 and S100A9) and tissue repair (annexin A1), which represent key events during EAE progression, were validated by quantitative PCR. This is the first targeted analysis of autoreactive cells purified from the CNS during EAE, highlighting fundamental CD4(+) cell-driven processes that occur during the initiation of relapse and remission stages of disease.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 4%
Korea, Republic of 1 4%
Unknown 24 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 23%
Student > Ph. D. Student 4 15%
Other 3 12%
Student > Master 3 12%
Student > Doctoral Student 2 8%
Other 6 23%
Unknown 2 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 27%
Medicine and Dentistry 5 19%
Agricultural and Biological Sciences 3 12%
Chemistry 2 8%
Nursing and Health Professions 1 4%
Other 6 23%
Unknown 2 8%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 April 2015.
All research outputs
#17,723,043
of 22,758,248 outputs
Outputs from Journal of Proteome Research
#4,890
of 6,021 outputs
Outputs of similar age
#154,895
of 227,590 outputs
Outputs of similar age from Journal of Proteome Research
#55
of 113 outputs
Altmetric has tracked 22,758,248 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 6,021 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one is in the 14th percentile – i.e., 14% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 227,590 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 113 others from the same source and published within six weeks on either side of this one. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.