| Title |
Pig transgenesis by piggyBac transposition in combination with somatic cell nuclear transfer
|
|---|---|
| Published in |
Transgenic Research, July 2013
|
| DOI | 10.1007/s11248-013-9729-0 |
| Pubmed ID | |
| Authors |
Zhenfang Wu, Zhiqian Xu, Xian Zou, Fang Zeng, Junsong Shi, Dewu Liu, Johann Urschitz, Stefan Moisyadi, Zicong Li |
| Abstract |
The production of animals by somatic cell nuclear transfer (SCNT) is inefficient, with approximately 2% of micromanipulated oocytes going to term and resulting in live births. However, it is the most commonly used method for the generation of cloned transgenic livestock as it facilitates the attainment of transgenic animals once the nuclear donor cells are stably transfected and more importantly as alternatives methods of transgenesis in farm animals have proven even less efficient. Here we describe piggyBac-mediated transposition of a transgene into porcine primary cells and use of these genetically modified cells as nuclear donors for the generation of transgenic pigs by SCNT. Gene transfer by piggyBac transposition serves to provide an alternative approach for the transfection of nuclear donor cells used in SCNT. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 22% |
| Researcher | 3 | 17% |
| Student > Postgraduate | 2 | 11% |
| Professor > Associate Professor | 2 | 11% |
| Student > Master | 1 | 6% |
| Other | 3 | 17% |
| Unknown | 3 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 33% |
| Biochemistry, Genetics and Molecular Biology | 4 | 22% |
| Chemistry | 2 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
| Unknown | 5 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 July 2014.
All research outputs
#20,232,430
of 22,758,248 outputs
Outputs from Transgenic Research
#814
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Outputs of similar age
#170,254
of 194,049 outputs
Outputs of similar age from Transgenic Research
#14
of 14 outputs
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