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Identification and characterisation of Kunitz-type plasma kallikrein inhibitors unique to Oxyuranus sp. snake venoms

Overview of attention for article published in Biochimie, August 2011
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Title
Identification and characterisation of Kunitz-type plasma kallikrein inhibitors unique to Oxyuranus sp. snake venoms
Published in
Biochimie, August 2011
DOI 10.1016/j.biochi.2011.08.003
Pubmed ID
Authors

Stephen T.H. Earl, Renee Richards, Lambro A. Johnson, Simone Flight, Steven Anderson, Ann Liao, John de Jersey, Paul P. Masci, Martin F. Lavin

Abstract

As part of a wider study on Australian snake venom components, we have identified and characterised Kunitz-type protease inhibitors from the venoms of Oxyuranus scutellatus and Oxyuranus microlepidotus (Australian taipans) with plasma kallikrein inhibitory activity. Each inhibitor had a mass of 7 kDa and was purified from the venom as part of a protein complex. Mass spectrometry and N-terminal sequencing was employed to obtain amino acid sequence information for each inhibitor and a recombinant form of the O. scutellatus inhibitor, termed TSPI, was subsequently expressed and purified. TSPI was investigated for inhibition against a panel of 12 enzymes involved in haemostasis and estimates of the K(i) value determined for each enzyme. TSPI was found to be a broad spectrum inhibitor with most potent inhibitory activity observed against plasma kallikrein that corresponded to a K(i) of 0.057 ± 0.019 nM. TSPI also inhibited fibrinolysis in whole blood and prolonged the intrinsic clotting time. These inhibitors are also unique in that they appear to be found only in Oxyuranus sp. venoms.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 2%
Unknown 42 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 23%
Student > Master 5 12%
Researcher 4 9%
Student > Bachelor 3 7%
Student > Doctoral Student 3 7%
Other 7 16%
Unknown 11 26%
Readers by discipline Count As %
Agricultural and Biological Sciences 22 51%
Biochemistry, Genetics and Molecular Biology 6 14%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Medicine and Dentistry 1 2%
Chemistry 1 2%
Other 2 5%
Unknown 10 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 August 2011.
All research outputs
#20,656,161
of 25,374,647 outputs
Outputs from Biochimie
#2,036
of 2,582 outputs
Outputs of similar age
#109,320
of 131,748 outputs
Outputs of similar age from Biochimie
#20
of 22 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
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We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.