| Title |
Artificial microRNA mediated gene silencing in plants: progress and perspectives
|
|---|---|
| Published in |
Plant Molecular Biology, July 2014
|
| DOI | 10.1007/s11103-014-0224-7 |
| Pubmed ID | |
| Authors |
Manish Tiwari, Deepika Sharma, Prabodh Kumar Trivedi |
| Abstract |
Homology based gene silencing has emerged as a convenient approach for repressing expression of genes in order to study their functions. For this purpose, several antisense or small interfering RNA based gene silencing techniques have been frequently employed in plant research. Artificial microRNAs (amiRNAs) mediated gene silencing represents one of such techniques which can utilize as a potential tool in functional genomics. Similar to microRNAs, amiRNAs are single-stranded, approximately 21 nt long, and designed by replacing the mature miRNA sequences of duplex within pre-miRNAs. These amiRNAs are processed via small RNA biogenesis and silencing machinery and deregulate target expression. Holding to various refinements, amiRNA technology offers several advantages over other gene silencing methods. This is a powerful and robust tool, and could be applied to unravel new insight of metabolic pathways and gene functions across the various disciplines as well as in translating observations for improving favourable traits in plants. This review highlights general background of small RNAs, improvements made in RNAi based gene silencing, implications of amiRNA in gene silencing, and describes future themes for improving value of this technology in plant science. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Argentina | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 249 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 3 | 1% |
| Turkey | 1 | <1% |
| India | 1 | <1% |
| Argentina | 1 | <1% |
| Belgium | 1 | <1% |
| Japan | 1 | <1% |
| United States | 1 | <1% |
| Philippines | 1 | <1% |
| Poland | 1 | <1% |
| Other | 0 | 0% |
| Unknown | 238 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 49 | 20% |
| Researcher | 43 | 17% |
| Student > Master | 34 | 14% |
| Student > Bachelor | 34 | 14% |
| Student > Doctoral Student | 16 | 6% |
| Other | 30 | 12% |
| Unknown | 43 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 119 | 48% |
| Biochemistry, Genetics and Molecular Biology | 54 | 22% |
| Computer Science | 3 | 1% |
| Chemistry | 2 | <1% |
| Immunology and Microbiology | 2 | <1% |
| Other | 12 | 5% |
| Unknown | 57 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 August 2023.
All research outputs
#3,433,644
of 24,413,320 outputs
Outputs from Plant Molecular Biology
#128
of 2,884 outputs
Outputs of similar age
#33,258
of 231,595 outputs
Outputs of similar age from Plant Molecular Biology
#1
of 20 outputs
Altmetric has tracked 24,413,320 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,884 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 95% of its peers.
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