Title |
Chemical Cell Lysis System Applicable to Lab-on-a-Disc
|
---|---|
Published in |
Applied Biochemistry and Biotechnology, February 2017
|
DOI | 10.1007/s12010-017-2428-1 |
Pubmed ID | |
Authors |
Dayeseul Lim, Jae Chern Yoo |
Abstract |
The design and fabrication of a heating system has been a significant challenge in implementing chemical lysis on a lab-on-a-disc (LOD). The proposed system contains a sample inlet, phase change material (PCM) array, heating chamber, and valve in a single disc, providing cost-effective, rapid, and fully automated chemical cell lysis. Compared to the conventional cell lysis system, our cell lysis system has many advantages, such as a compact structure that is easily integrated into the LOD and reduced processing time and labor. The experiments are conducted with Salmonella typhimurium strains to demonstrate the performance. The experimental results show that the proposed approach is greatly effective in realizing a chemical cell lysis system on an LOD with higher throughput in terms of purity and yield of DNA. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 13% |
Researcher | 2 | 13% |
Student > Bachelor | 2 | 13% |
Unspecified | 1 | 7% |
Unknown | 8 | 53% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 13% |
Chemical Engineering | 1 | 7% |
Unspecified | 1 | 7% |
Immunology and Microbiology | 1 | 7% |
Social Sciences | 1 | 7% |
Other | 2 | 13% |
Unknown | 7 | 47% |