| Title |
In vitro characterization of scaffold-free three-dimensional mesenchymal stem cell aggregates
|
|---|---|
| Published in |
Cell and Tissue Research, July 2014
|
| DOI | 10.1007/s00441-014-1939-0 |
| Pubmed ID | |
| Authors |
Christina L. Rettinger, Andrea B. Fourcaudot, Seok J. Hong, Thomas A. Mustoe, Robert G. Hale, Kai P. Leung |
| Abstract |
Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation along multiple cell lineages and have potential applications in a wide range of therapies. These cells are commonly cultured as monolayers on tissue culture plastic but possibly lose their cell-specific properties with time in vitro. There is growing interest in culturing adherent cells via three-dimensional (3D) techniques in order to recapitulate 3D in vivo conditions. We describe a novel method for generating and culturing rabbit MSCs as scaffold-free 3D cell aggregates by using micropatterned wells via a forced aggregation technique. The viability and proliferative capability of MSC aggregates were assessed via Live/Dead staining and 5-ethynyl-2'-deoxyuridine (EdU) incorporation. Enzyme-linked immunosorbent assay and antibody-based multiplex protein assays were used to quantify released growth factors and chemokines. The gene expression profile of MSCs as 3D aggregates relative to MSCs grown as monolayers was evaluated via quantitative real-time polymerase chain reaction. The rabbit MSCs were able to form compact cell aggregates and remained viable in 3D culture for up to 7 days. We also demonstrated enhanced gene and protein expression related to angiogenesis and wound healing in MSCs cultured under 3D conditions. In vitro tube formation and scratch assay revealed superior neovessel formation and greater cell recovery and migration in response to 3D conditioned media after wounding. Our data further suggest that adipose-derived stem cell aggregates have greater potential than dermal fibroblasts or bone-marrow-derived MSCs in accelerating wound healing and reducing scarring. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 17% |
| Saudi Arabia | 1 | 17% |
| Unknown | 4 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 5 | 83% |
| Science communicators (journalists, bloggers, editors) | 1 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 70 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 3% |
| Canada | 2 | 3% |
| China | 1 | 1% |
| United States | 1 | 1% |
| Unknown | 64 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 14 | 20% |
| Student > Bachelor | 12 | 17% |
| Student > Master | 8 | 11% |
| Researcher | 7 | 10% |
| Student > Doctoral Student | 5 | 7% |
| Other | 15 | 21% |
| Unknown | 9 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 13 | 19% |
| Agricultural and Biological Sciences | 13 | 19% |
| Medicine and Dentistry | 12 | 17% |
| Biochemistry, Genetics and Molecular Biology | 8 | 11% |
| Chemical Engineering | 3 | 4% |
| Other | 11 | 16% |
| Unknown | 10 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 June 2022.
All research outputs
#5,700,702
of 23,839,820 outputs
Outputs from Cell and Tissue Research
#240
of 2,279 outputs
Outputs of similar age
#47,469
of 228,392 outputs
Outputs of similar age from Cell and Tissue Research
#4
of 30 outputs
Altmetric has tracked 23,839,820 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,279 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 228,392 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 77% of its contemporaries.
We're also able to compare this research output to 30 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.