| Title |
Phenotypic and genetic characterization of Vibrio cholerae O1 clinical isolates collected through national antimicrobial resistance surveillance network in Nepal
|
|---|---|
| Published in |
World Journal of Microbiology and Biotechnology, May 2012
|
| DOI | 10.1007/s11274-012-1077-3 |
| Pubmed ID | |
| Authors |
Geeta Shakya, Dong Wook Kim, John D. Clemens, Sarala Malla, Bishnu Prasad Upadhyaya, Shyam Prakash Dumre, Sirjana Devi Shrestha, Shailaja Adhikari, Supriya Sharma, Nisha Rijal, Sanjaya K. Shrestha, Carl Mason, Palpasa Kansakar |
| Abstract |
Cholera occurs in sporadic cases and outbreaks in Nepal each year. Vibrio cholerae O1 (n = 522) isolated during 2007-2010 from diarrheal patients at 10 different hospital laboratories in Nepal were characterized. Biochemical and serologic identifications showed that all the isolates belonged to serogroup O1, El Tor biotype. Except 72 isolates of Inaba serotype isolated in the year 2007, all the remaining isolates were of Ogawa serotype. All isolates were resistant to nalidixic acid and furazolidone. Resistance to tetracycline, ciprofloxacin, erythromycin and co-trimoxazole were 21, 4, 16 and 90 % respectively. Seventy-seven of these isolates were selected for further characterization for ctxB gene and MLVA typing. Two different variants of classical type cholera toxin were observed. Ogawa strains from 2007 and 2010-Western Nepal outbreak harbored CTX-3 type cholera toxin, whereas Inaba serotypes in 2007 and the remaining Ogawa serotypes in 2008-2010 harbored CTX 3b-type toxin. MLVA analysis showed circulation of four different groups of altered V. cholerae O1 El Tor strains. Two different profiles were seen among 2007 Inaba (9, 3, 6, x, x) and Ogawa (10, 7, 6, x, x) isolates. The MLVA profile of 2008 and 2009 Ogawa isolates were similar to those of Inaba strains of 2007. Isolates from 2010 also showed three different MLVA profiles; profile 9, 3, 6, x, x in 3 isolates, 11, 7, 6, x, x among 2010 Western Nepal outbreak strains and profile 8, 3, 6, x, x among isolates from Butwal and Kathmandu. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Nepal | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 30 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 20% |
| Student > Master | 4 | 13% |
| Student > Postgraduate | 3 | 10% |
| Student > Doctoral Student | 2 | 7% |
| Student > Bachelor | 2 | 7% |
| Other | 3 | 10% |
| Unknown | 10 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 20% |
| Medicine and Dentistry | 5 | 17% |
| Environmental Science | 2 | 7% |
| Veterinary Science and Veterinary Medicine | 2 | 7% |
| Immunology and Microbiology | 2 | 7% |
| Other | 4 | 13% |
| Unknown | 9 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 July 2014.
All research outputs
#16,223,992
of 23,911,072 outputs
Outputs from World Journal of Microbiology and Biotechnology
#990
of 1,757 outputs
Outputs of similar age
#107,426
of 166,786 outputs
Outputs of similar age from World Journal of Microbiology and Biotechnology
#11
of 16 outputs
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