| Title |
Biochemical and molecular characterization of Cronobacter spp. (formerly Enterobacter sakazakii) isolated from foods
|
|---|---|
| Published in |
Antonie van Leeuwenhoek, July 2010
|
| DOI | 10.1007/s10482-010-9484-7 |
| Pubmed ID | |
| Authors |
Imrich Turcovský, Kristína Kuniková, Hana Drahovská, Eva Kaclíková |
| Abstract |
The aim of this study was to identify and characterize Cronobacter spp. isolated from a range of foods. A total of 71 Cronobacter strains were isolated from 602 foods in our laboratory. The highest contamination was observed in foods of plant origin, e.g. spices, teas, chocolate, nuts, pastries and vegetables. On the basis of genus and species identification performed using genus-specific PCR, 16S rRNA sequencing and AFLP genotyping, most of the strains belonged to Cronobacter sakazakii. Biochemical profiling by the tests included in API 20E, complemented with relevant additional tests, classified the strains into 13 biogroups. AFLP genotyping facilitated discrimination of six main groups at the 70% similarity level and strain grouping correlated clearly with species identification. Our results indicate that molecular typing by AFLP may be applied as a useful tool not only for direct comparison of Cronobacter isolates, providing traceability, but also for the reliable species classification. Moreover, tracing of these bacteria in a wider variety of foods should be important to enhance the knowledge of their transmission. |
Mendeley readers
The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 39 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 18% |
| Student > Ph. D. Student | 6 | 15% |
| Student > Master | 5 | 13% |
| Student > Bachelor | 3 | 8% |
| Student > Postgraduate | 3 | 8% |
| Other | 7 | 18% |
| Unknown | 8 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 41% |
| Immunology and Microbiology | 7 | 18% |
| Biochemistry, Genetics and Molecular Biology | 6 | 15% |
| Environmental Science | 1 | 3% |
| Earth and Planetary Sciences | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 8 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 October 2022.
All research outputs
#3,480,951
of 23,917,011 outputs
Outputs from Antonie van Leeuwenhoek
#158
of 2,074 outputs
Outputs of similar age
#11,645
of 83,867 outputs
Outputs of similar age from Antonie van Leeuwenhoek
#1
of 10 outputs
Altmetric has tracked 23,917,011 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,074 research outputs from this source. They receive a mean Attention Score of 3.7. This one has done particularly well, scoring higher than 91% of its peers.
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