| Title |
Role of Src in breast cancer cell migration and invasion in a breast cell/bone-derived cell microenvironment
|
|---|---|
| Published in |
Breast Cancer Research and Treatment, September 2011
|
| DOI | 10.1007/s10549-011-1753-2 |
| Pubmed ID | |
| Authors |
Brant Pohorelic, R. Singh, S. Parkin, K. Koro, A.-D. Yang, C. Egan, A. Magliocco |
| Abstract |
The preferential metastasis of breast cancer cells to bone comprises a complex set of events including homing and preferential growth, which may require unique factors produced by bone or other cells in the immediate microenvironment. In this study, an in vitro co-culture system composed of bone mesenchymal stem cells and breast cancer cell lines is used to examine the role of Src kinase on breast cancer cell migration and invasion in the presence of bone-derived cells. This research shows that Src kinase activity in breast cancer cell lines with either high or low levels of endogenous Src activity is increased by bone-derived cell-conditioned medium but not HS68 fibroblast-conditioned medium. Breast cancer cells exhibit enhanced migration in co-culture with bone-derived cells but not HS68 fibroblasts or no co-cultured cells. Inhibition of Src kinase activity using the inhibitors PP2 or saracatinib or using siRNA abrogates the preferential migration of the breast cancer cell lines in response to bone-derived cells. Inhibition of Src activity with saracatinib does not have any significant effect on breast cancer cell invasion in the presence of bone-derived cells. Factors are identified that are produced preferentially by bone-derived cells over HS68 cells that may impact breast cancer cell behavior. This research implicates Src kinase as an important effector of bone-derived cell signals on breast cancer cell migration. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 2% |
| Unknown | 40 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 32% |
| Student > Master | 5 | 12% |
| Researcher | 5 | 12% |
| Student > Bachelor | 4 | 10% |
| Other | 2 | 5% |
| Other | 6 | 15% |
| Unknown | 6 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 37% |
| Biochemistry, Genetics and Molecular Biology | 6 | 15% |
| Medicine and Dentistry | 6 | 15% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 5% |
| Social Sciences | 1 | 2% |
| Other | 2 | 5% |
| Unknown | 9 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 September 2011.
All research outputs
#18,295,723
of 22,651,245 outputs
Outputs from Breast Cancer Research and Treatment
#3,678
of 4,612 outputs
Outputs of similar age
#103,879
of 125,614 outputs
Outputs of similar age from Breast Cancer Research and Treatment
#40
of 47 outputs
Altmetric has tracked 22,651,245 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,612 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.2. This one is in the 11th percentile – i.e., 11% of its peers scored the same or lower than it.
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