| Title |
BSE infectivity in jejunum, ileum and ileocaecal junction of incubating cattle
|
|---|---|
| Published in |
Veterinary Research, February 2011
|
| DOI | 10.1186/1297-9716-42-21 |
| Pubmed ID | |
| Authors |
Christine Hoffmann, Martin Eiden, Martin Kaatz, Markus Keller, Ute Ziegler, Ron Rogers, Bob Hills, Anne Balkema-Buschmann, Lucien van Keulen, Jorg G Jacobs, Martin H Groschup |
| Abstract |
To establish bovine spongiform encephalopathy (BSE) public health protection measures it is important to precisely define the cattle tissues considered as specified risk materials (SRM). To date, in pre-clinical BSE infected cattle, no evidence of the BSE agent had been found in the gut outside of the ileal Peyer's Patches. This study was undertaken to determine when and where the pathological prion protein (PrPSc) and/or BSE infectivity can be found in the small intestine of cattle 4 to 6 months of age, orally challenged with BSE. Samples of the jejunum, the ileum and the ileocaecal junction from 46 BSE infected cattle, culled from 1 up to 44 months post infection (mpi) were examined by immunohistochemistry. Samples from cattle 8 mpi to 20 mpi were additionally studied by PTA Western blot, rapid tests, and by mouse (TgbovXV) bioassay. In doing so nearly all of the cattle, from 4 up to 44 mpi, had detectable amounts of PrPSc and/or infectivity in the distal ileum. In the distal ileum clear time-dependent variations were visible concerning the amount of PrPSc, the tissue structures affected, and the cells involved. BSE infectivity was found not only in the ileum and ileocaecal junction but also in the jejunum. The systematic approach of this study provides new data for qualitative and quantitative risk assessments and allows defining bovine SRM more precisely. |
Mendeley readers
The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 3% |
| Poland | 1 | 3% |
| Unknown | 27 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 28% |
| Student > Ph. D. Student | 7 | 24% |
| Professor | 3 | 10% |
| Other | 2 | 7% |
| Student > Master | 2 | 7% |
| Other | 4 | 14% |
| Unknown | 3 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 34% |
| Biochemistry, Genetics and Molecular Biology | 6 | 21% |
| Veterinary Science and Veterinary Medicine | 4 | 14% |
| Immunology and Microbiology | 2 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Other | 4 | 14% |
| Unknown | 2 | 7% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2014.
All research outputs
#5,447,195
of 25,374,917 outputs
Outputs from Veterinary Research
#245
of 1,337 outputs
Outputs of similar age
#34,745
of 194,759 outputs
Outputs of similar age from Veterinary Research
#10
of 23 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,337 research outputs from this source. They receive a mean Attention Score of 5.0. This one has done well, scoring higher than 78% of its peers.
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We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.