| Title |
Simultaneous determination of 186 fungal and bacterial metabolites in indoor matrices by liquid chromatography/tandem mass spectrometry
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, August 2009
|
| DOI | 10.1007/s00216-009-2995-2 |
| Pubmed ID | |
| Authors |
Vinay Vishwanath, Michael Sulyok, Roman Labuda, Wolfgang Bicker, Rudolf Krska |
| Abstract |
This paper describes the application of a previously published multi-mycotoxin method for food and feed matrices based on liquid chromatography/electrospray ionization-tandem mass spectrometry (HPLC/ESI-MS/MS) to the analysis of microbial metabolites in indoor matrices. The range of investigated analytes has been extended by 99 fungal and bacterial metabolites to cover now 186 compounds overall. The method is based on a single extraction step using an acidified acetonitrile/water mixture (which has been determined to be preferable to methanol and ethyl acetate) followed by analysis of the diluted crude extract. The analytical signal of one third of the investigated analytes was reduced by more than 50% due to matrix effects in a spiked extract of house dust, whereas the other investigated materials were less critical in that aspect. For determination of method performance characteristics, a spiked reference material for house dust was chosen as a model sample for an extremely complex matrix. With few exceptions, coefficients of variation of the whole procedure of <10% and limits of detection of <50 microg kg(-1) were obtained. The apparent recoveries were below 50% for half of the investigated analytes due to incomplete extraction and/or detection-related matrix effects. The application of the method to 14 samples from damp buildings revealed the presence of 20 different analytes at concentrations of up to 130 mg kg(-1). Most of these compounds have never been identified before in real-world samples, although they are known to be produced by indoor-relevant fungi. This underlines the great value of the described method for the on-site determination of microbial metabolites. |
Mendeley readers
The data shown below were compiled from readership statistics for 108 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 2 | 2% |
| Croatia | 1 | <1% |
| Austria | 1 | <1% |
| India | 1 | <1% |
| Unknown | 103 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 27 | 25% |
| Student > Ph. D. Student | 20 | 19% |
| Student > Master | 18 | 17% |
| Student > Doctoral Student | 7 | 6% |
| Student > Bachelor | 5 | 5% |
| Other | 15 | 14% |
| Unknown | 16 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 35 | 32% |
| Chemistry | 19 | 18% |
| Biochemistry, Genetics and Molecular Biology | 13 | 12% |
| Medicine and Dentistry | 4 | 4% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 3% |
| Other | 8 | 7% |
| Unknown | 26 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 January 2014.
All research outputs
#2,863,832
of 25,373,627 outputs
Outputs from Analytical & Bioanalytical Chemistry
#239
of 9,619 outputs
Outputs of similar age
#10,439
of 123,507 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#4
of 52 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,619 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done particularly well, scoring higher than 97% of its peers.
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We're also able to compare this research output to 52 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.