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Gene Expression Profiling

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Cover of 'Gene Expression Profiling'

Table of Contents

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    Book Overview
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    Chapter 1 Real-Time PCR and Multiplex Approaches
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    Chapter 2 Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs
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    Chapter 3 Microarray Analysis of mRNAs: Experimental Design and Data Analysis Fundamentals
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    Chapter 4 Software and Tools for Microarray Data Analysis
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    Chapter 5 Analysis of Gene Expression as Relevant to Cancer Cells and Circulating Tumour Cells
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    Chapter 6 Gene Expression Profiling in Formalin-Fixed, Paraffin-Embedded Tissues Using the Whole-Genome DASL Assay
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    Chapter 7 MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs.
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    Chapter 8 Western Blotting Analysis as a Tool to Study Receptor Tyrosine Kinases
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    Chapter 9 2D Gel Electrophoresis and Mass Spectrometry Identification and Analysis of Proteins
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    Chapter 10 Gene Expression Profiling
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    Chapter 11 Immunohistochemical and Immunofluorescence Procedures for Protein Analysis
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    Chapter 12 Advanced Microscopy: Laser Scanning Confocal Microscopy
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    Chapter 13 Isolation of Exosomes for Subsequent mRNA, MicroRNA, and Protein Profiling.
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    Chapter 14 Atomic Force Microscopy and High-Content Analysis: Two Innovative Technologies for Dissecting the Relationship Between Epithelial–Mesenchymal Transition-Related Morphological and Structural Alterations and Cell Mechanical Properties
Attention for Chapter 7: MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs.
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Chapter title
MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs.
Chapter number 7
Book title
Gene Expression Profiling
Published in
Methods in molecular biology, January 2011
DOI 10.1007/978-1-61779-289-2_7
Pubmed ID
Book ISBNs
978-1-61779-288-5, 978-1-61779-289-2
Authors

Erica Hennessy, Lorraine O’Driscoll, O'DRISCOLL, LORRAINE, Hennessy, Erica, O’Driscoll, Lorraine

Abstract

MicroRNAs, the class of small ribo-regulators, have been implicated in the regulation of a range of different biological processes, including development and differentiation, proliferation, and cell death. Only for a small fraction of identified microRNAs has a function been elucidated; therefore, a great deal of research remains to be performed to fully understand the role and implications of microRNAs.This chapter discusses protocols for the isolation of microRNAs, reverse transcription, PCR, and large scale profiling using TaqMan low density miRNA arrays for analysis of microRNA expression levels.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 10%
Unknown 9 90%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 40%
Student > Bachelor 3 30%
Lecturer 1 10%
Student > Ph. D. Student 1 10%
Student > Postgraduate 1 10%
Other 0 0%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 60%
Agricultural and Biological Sciences 3 30%
Unknown 1 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 September 2011.
All research outputs
#18,295,723
of 22,651,245 outputs
Outputs from Methods in molecular biology
#7,803
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Outputs of similar age
#159,884
of 180,239 outputs
Outputs of similar age from Methods in molecular biology
#166
of 229 outputs
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