Title |
Comparison of gene expression microarray data with count-based RNA measurements informs microarray interpretation
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Published in |
BMC Genomics, August 2014
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DOI | 10.1186/1471-2164-15-649 |
Pubmed ID | |
Authors |
Arianne C Richard, Paul A Lyons, James E Peters, Daniele Biasci, Shaun M Flint, James C Lee, Eoin F McKinney, Richard M Siegel, Kenneth GC Smith |
Abstract |
Although numerous investigations have compared gene expression microarray platforms, preprocessing methods and batch correction algorithms using constructed spike-in or dilution datasets, there remains a paucity of studies examining the properties of microarray data using diverse biological samples. Most microarray experiments seek to identify subtle differences between samples with variable background noise, a scenario poorly represented by constructed datasets. Thus, microarray users lack important information regarding the complexities introduced in real-world experimental settings. The recent development of a multiplexed, digital technology for nucleic acid measurement enables counting of individual RNA molecules without amplification and, for the first time, permits such a study. |
X Demographics
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France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 4 | 4% |
United Kingdom | 3 | 3% |
Hong Kong | 1 | <1% |
Netherlands | 1 | <1% |
Finland | 1 | <1% |
Unknown | 96 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 29 | 27% |
Student > Ph. D. Student | 18 | 17% |
Student > Bachelor | 16 | 15% |
Student > Master | 13 | 12% |
Other | 5 | 5% |
Other | 19 | 18% |
Unknown | 6 | 6% |
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Biochemistry, Genetics and Molecular Biology | 19 | 18% |
Medicine and Dentistry | 15 | 14% |
Computer Science | 4 | 4% |
Mathematics | 3 | 3% |
Other | 8 | 8% |
Unknown | 12 | 11% |