| Title |
Cannabidiol Exposure During Neuronal Differentiation Sensitizes Cells Against Redox-Active Neurotoxins
|
|---|---|
| Published in |
Molecular Neurobiology, August 2014
|
| DOI | 10.1007/s12035-014-8843-1 |
| Pubmed ID | |
| Authors |
Patrícia Schönhofen, Liana M. de Medeiros, Ivi Juliana Bristot, Fernanda M. Lopes, Marco A. De Bastiani, Flávio Kapczinski, José Alexandre S. Crippa, Mauro Antônio A. Castro, Richard B. Parsons, Fábio Klamt |
| Abstract |
Cannabidiol (CBD), one of the most abundant Cannabis sativa-derived compounds, has been implicated with neuroprotective effect in several human pathologies. Until now, no undesired side effects have been associated with CBD. In this study, we evaluated CBD's neuroprotective effect in terminal differentiation (mature) and during neuronal differentiation (neuronal developmental toxicity model) of the human neuroblastoma SH-SY5Y cell line. A dose-response curve was performed to establish a sublethal dose of CBD with antioxidant activity (2.5 μM). In terminally differentiated SH-SY5Y cells, incubation with 2.5 μM CBD was unable to protect cells against the neurotoxic effect of glycolaldehyde, methylglyoxal, 6-hydroxydopamine, and hydrogen peroxide (H2O2). Moreover, no difference in antioxidant potential and neurite density was observed. When SH-SY5Y cells undergoing neuronal differentiation were exposed to CBD, no differences in antioxidant potential and neurite density were observed. However, CBD potentiated the neurotoxicity induced by all redox-active drugs tested. Our data indicate that 2.5 μM of CBD, the higher dose tolerated by differentiated SH-SY5Y neuronal cells, does not provide neuroprotection for terminally differentiated cells and shows, for the first time, that exposure of CBD during neuronal differentiation could sensitize immature cells to future challenges with neurotoxins. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 99 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 1% |
| Unknown | 98 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 17 | 17% |
| Student > Bachelor | 15 | 15% |
| Researcher | 11 | 11% |
| Student > Ph. D. Student | 11 | 11% |
| Professor | 8 | 8% |
| Other | 15 | 15% |
| Unknown | 22 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 18% |
| Pharmacology, Toxicology and Pharmaceutical Science | 10 | 10% |
| Biochemistry, Genetics and Molecular Biology | 9 | 9% |
| Medicine and Dentistry | 9 | 9% |
| Neuroscience | 6 | 6% |
| Other | 22 | 22% |
| Unknown | 25 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 September 2020.
All research outputs
#14,198,374
of 22,759,618 outputs
Outputs from Molecular Neurobiology
#1,810
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Outputs of similar age
#118,882
of 230,421 outputs
Outputs of similar age from Molecular Neurobiology
#18
of 33 outputs
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