Title |
A microdevice for the creation of patent, three-dimensional endothelial cell-based microcirculatory networks
|
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Published in |
Biomicrofluidics, August 2011
|
DOI | 10.1063/1.3609264 |
Pubmed ID | |
Authors |
Lien T. Chau, Barbara E. Rolfe, Justin J. Cooper-White |
Abstract |
Microvascular network formation is a significant and challenging goal in the engineering of large three-dimensional artificial tissue structures. We show here the development of a fully patent, 3D endothelial cell (microvascular) microfluidic network that has a single inlet and outlet, created in only 28 h in a microdevice involving fluid flow equivalent to natural vasculature. Our microdevice features a tailored "multi-rung ladder" network, a stylized mimic of an arterial-to-venous pedicle, designed to also allow for systematic and reproducible cell seeding. Immunofluorescence staining revealed a highly contiguous endothelial monolayer (human umbilical vein endothelial cells) throughout the whole network after 24 h of continuous perfusion. This network persisted for up to 72 h of culture, providing a useful template from which the effects of surface chemistry, fluid flow, and environmental conditions on the development of artificial vascular networks ex vivo may be rapidly and robustly evaluated. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Australia | 1 | 4% |
Unknown | 22 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 43% |
Researcher | 5 | 22% |
Student > Master | 3 | 13% |
Student > Doctoral Student | 2 | 9% |
Student > Bachelor | 1 | 4% |
Other | 1 | 4% |
Unknown | 1 | 4% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 7 | 30% |
Biochemistry, Genetics and Molecular Biology | 3 | 13% |
Computer Science | 3 | 13% |
Agricultural and Biological Sciences | 2 | 9% |
Business, Management and Accounting | 1 | 4% |
Other | 2 | 9% |
Unknown | 5 | 22% |