Title |
Comprehensive analysis of the chromatin landscape in Drosophila melanogaster
|
---|---|
Published in |
Nature, December 2010
|
DOI | 10.1038/nature09725 |
Pubmed ID | |
Authors |
Peter V. Kharchenko, Artyom A. Alekseyenko, Yuri B. Schwartz, Aki Minoda, Nicole C. Riddle, Jason Ernst, Peter J. Sabo, Erica Larschan, Andrey A. Gorchakov, Tingting Gu, Daniela Linder-Basso, Annette Plachetka, Gregory Shanower, Michael Y. Tolstorukov, Lovelace J. Luquette, Ruibin Xi, Youngsook L. Jung, Richard W. Park, Eric P. Bishop, Theresa K. Canfield, Richard Sandstrom, Robert E. Thurman, David M. MacAlpine, John A. Stamatoyannopoulos, Manolis Kellis, Sarah C. R. Elgin, Mitzi I. Kuroda, Vincenzo Pirrotta, Gary H. Karpen, Peter J. Park |
Abstract |
Chromatin is composed of DNA and a variety of modified histones and non-histone proteins, which have an impact on cell differentiation, gene regulation and other key cellular processes. Here we present a genome-wide chromatin landscape for Drosophila melanogaster based on eighteen histone modifications, summarized by nine prevalent combinatorial patterns. Integrative analysis with other data (non-histone chromatin proteins, DNase I hypersensitivity, GRO-Seq reads produced by engaged polymerase, short/long RNA products) reveals discrete characteristics of chromosomes, genes, regulatory elements and other functional domains. We find that active genes display distinct chromatin signatures that are correlated with disparate gene lengths, exon patterns, regulatory functions and genomic contexts. We also demonstrate a diversity of signatures among Polycomb targets that include a subset with paused polymerase. This systematic profiling and integrative analysis of chromatin signatures provides insights into how genomic elements are regulated, and will serve as a resource for future experimental investigations of genome structure and function. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Canada | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 46 | 4% |
United Kingdom | 15 | 1% |
Spain | 11 | <1% |
France | 8 | <1% |
Germany | 6 | <1% |
Japan | 6 | <1% |
Sweden | 3 | <1% |
Austria | 3 | <1% |
Brazil | 3 | <1% |
Other | 31 | 3% |
Unknown | 1044 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 341 | 29% |
Researcher | 309 | 26% |
Student > Master | 94 | 8% |
Professor > Associate Professor | 78 | 7% |
Student > Bachelor | 68 | 6% |
Other | 177 | 15% |
Unknown | 109 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 676 | 57% |
Biochemistry, Genetics and Molecular Biology | 252 | 21% |
Computer Science | 36 | 3% |
Medicine and Dentistry | 26 | 2% |
Neuroscience | 16 | 1% |
Other | 48 | 4% |
Unknown | 122 | 10% |