| Title |
Induction of diabetes by Streptozotocin in rats
|
|---|---|
| Published in |
Indian Journal of Clinical Biochemistry, September 2007
|
| DOI | 10.1007/bf02913315 |
| Pubmed ID | |
| Authors |
A. Akbarzadeh, D. Norouzian, M. R. Mehrabi, Sh. Jamshidi, A. Farhangi, A. Allah Verdi, S. M. A. Mofidian, B. Lame Rad |
| Abstract |
The objective of this study is to induce experimental diabetes mellitus by Streptozotocin in normal adult Wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and C-peptide in serum, between normal and diabetic rats. Intra-venous injection of 60mg/kg dose of Streptozotocin in adult wistar rats, makes pancreas swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes mellitus in the 2-4 days. Induction of experimental diabetes mellitus is indeed the first step in the plan of purification of pancreatic Langerhans islet cells of normal rats for transplanting under the testis subcutaneous of experimentally induced diabetic rats. Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with non-ketosis hyperglycemia in some animal species. For induction of experimental diabetes in male adult rats weighted 250-300 grams (75-90 days), 60mg/kg of Streptozotocin was injected intravenously. Three days after degeneration of beta cells, diabetes was induced in all animals. The diabetic and normal animals were kept in the metabolic cages separately and their body weight, consumption of food and water, urine volume, the levels of serum glucose, insulin and C-peptide quantities in all animals were measured and then these quantities were compared. For a microscopic study of degeneration of Langerhans islet beta cells of diabetic rats, sampling from pancreas tissue of diabetic and normal rats, staining and comparison between them, were done. Induction of diabetes with Streptozotocin decreases Nicotinamide-adenine dinucleotide (NAD) in pancreas islet beta cells and causes histopathological effects in beta cells which probably intermediates induction of diabetes. In this study, we used Streptozotocin for our experiments in induction of experimental diabetes mellitus. After Induction of diabetes, consumption of food and water, volume of urine and glucose increased in the diabetic animals in comparison with normal animals, but the weight of body and the volume of insulin and C-peptide decreased in the diabetic animals. Sampling and staining of pancreas tissue of diabetic and normal rats showed that the Langerhans islet beta cells of diabetic rats have been clearly degenerated. In three days, Streptozotocin makes pancreas swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes. It also changes normal metabolism in diabetic rats in comparison with normal rats. Consumption of water and food, volume of urine, serum glucose increases in diabetic animals in comparison with normal rats but the levels of serum insulin, C-peptide and body weight decreases. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 995 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 5 | <1% |
| India | 4 | <1% |
| United States | 2 | <1% |
| Indonesia | 1 | <1% |
| Malaysia | 1 | <1% |
| Iran, Islamic Republic of | 1 | <1% |
| Turkey | 1 | <1% |
| Spain | 1 | <1% |
| Belgium | 1 | <1% |
| Other | 0 | 0% |
| Unknown | 978 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 162 | 16% |
| Student > Master | 151 | 15% |
| Student > Ph. D. Student | 133 | 13% |
| Researcher | 76 | 8% |
| Student > Postgraduate | 50 | 5% |
| Other | 139 | 14% |
| Unknown | 284 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 166 | 17% |
| Agricultural and Biological Sciences | 160 | 16% |
| Biochemistry, Genetics and Molecular Biology | 118 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 107 | 11% |
| Neuroscience | 26 | 3% |
| Other | 115 | 12% |
| Unknown | 303 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 April 2023.
All research outputs
#4,764,915
of 23,760,369 outputs
Outputs from Indian Journal of Clinical Biochemistry
#68
of 381 outputs
Outputs of similar age
#13,236
of 70,168 outputs
Outputs of similar age from Indian Journal of Clinical Biochemistry
#2
of 8 outputs
Altmetric has tracked 23,760,369 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 381 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.5. This one has done well, scoring higher than 82% of its peers.
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