| Title |
SIRT6 knockout cells resist apoptosis initiation but not progression: a computational method to evaluate the progression of apoptosis
|
|---|---|
| Published in |
Apoptosis, August 2017
|
| DOI | 10.1007/s10495-017-1412-0 |
| Pubmed ID | |
| Authors |
Sergii Domanskyi, Justin W. Nicholatos, Joshua E. Schilling, Vladimir Privman, Sergiy Libert |
| Abstract |
Apoptosis is essential for numerous processes, such as development, resistance to infections, and suppression of tumorigenesis. Here, we investigate the influence of the nutrient sensing and longevity-assuring enzyme SIRT6 on the dynamics of apoptosis triggered by serum starvation. Specifically, we characterize the progression of apoptosis in wild type and SIRT6 deficient mouse embryonic fibroblasts using time-lapse flow cytometry and computational modelling based on rate-equations and cell distribution analysis. We find that SIRT6 deficient cells resist apoptosis by delaying its initiation. Interestingly, once apoptosis is initiated, the rate of its progression is higher in SIRT6 null cells compared to identically cultured wild type cells. However, SIRT6 null cells succumb to apoptosis more slowly, not only in response to nutrient deprivation but also in response to other stresses. Our data suggest that SIRT6 plays a role in several distinct steps of apoptosis. Overall, we demonstrate the utility of our computational model to describe stages of apoptosis progression and the integrity of the cellular membrane. Such measurements will be useful in a broad range of biological applications. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 43% |
| Student > Bachelor | 1 | 14% |
| Researcher | 1 | 14% |
| Student > Master | 1 | 14% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 29% |
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 14% |
| Nursing and Health Professions | 1 | 14% |
| Medicine and Dentistry | 1 | 14% |
| Other | 0 | 0% |
| Unknown | 1 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 October 2017.
All research outputs
#17,915,942
of 23,002,898 outputs
Outputs from Apoptosis
#517
of 809 outputs
Outputs of similar age
#226,382
of 315,730 outputs
Outputs of similar age from Apoptosis
#3
of 13 outputs
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So far Altmetric has tracked 809 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
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We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 76% of its contemporaries.