| Title |
Characterization of Arabidopsis Tubby-like proteins and redundant function of AtTLP3 and AtTLP9 in plant response to ABA and osmotic stress
|
|---|---|
| Published in |
Plant Molecular Biology, August 2014
|
| DOI | 10.1007/s11103-014-0241-6 |
| Pubmed ID | |
| Authors |
Yan Bao, Wei-Meng Song, Yan-Li Jin, Chun-Mei Jiang, Yang Yang, Bei Li, Wei-Jie Huang, Hua Liu, Hong-Xia Zhang |
| Abstract |
Tubby and Tubby-like proteins (TLPs) play essential roles in the development and function of mammal neuronal cells. In addition to the conserved carboxyl (C)-terminal Tubby domain, which is required for their plasma membrane (PM) tethering, plant TLPs also possess an amino (N)-terminal F-box domain to interact with specific Arabidopsis Skp1-like (ASK) proteins as functional SCF-type E3 ligases. Here, we report the molecular characterization of Arabidopsis TLPs (AtTLPs). β-Glucuronidase staining showed overlapped but distinct expression patterns of AtTLPs in Arabidopsis. Yeast two-hybrid assays further revealed that AtTLP1, AtTLP3, AtTLP6, AtTLP7, AtTLP9, AtTLP10 and AtTLP11 all interacted with specific ASKs, but AtTLP2, AtTLP5 and AtTLP8 did not. Subcellular localization observations in both Arabidopsis protoplasts and tobacco pollen tubes indicated that all GFP-AtTLP fusion proteins, except GFP-AtTLP8 which lacks the conserved phosphatidylinositol 4,5-bisphosphate binding sites, were targeted to the PM. Detailed studies on AtTLP3 demonstrated that AtTLP3 is a PM-tethered PIP2 binding protein which functions redundantly with AtTLP9 in abscisic acid (ABA)- and osmotic stress-mediated seed germination. Our results suggest that AtTLPs possibly work in multiple physiological and developmental processes in Arabidopsis, and AtTLP3 is also involved in ABA signaling pathway like AtTLP9 during seed germination and early seedling growth. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 61 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 18% |
| Researcher | 11 | 18% |
| Student > Master | 11 | 18% |
| Student > Bachelor | 6 | 10% |
| Student > Doctoral Student | 4 | 7% |
| Other | 7 | 11% |
| Unknown | 11 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 30 | 49% |
| Biochemistry, Genetics and Molecular Biology | 14 | 23% |
| Unspecified | 2 | 3% |
| Nursing and Health Professions | 1 | 2% |
| Chemical Engineering | 1 | 2% |
| Other | 2 | 3% |
| Unknown | 11 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 August 2014.
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#18,376,927
of 22,761,738 outputs
Outputs from Plant Molecular Biology
#2,492
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Outputs of similar age
#168,260
of 236,210 outputs
Outputs of similar age from Plant Molecular Biology
#8
of 21 outputs
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