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X Demographics
Mendeley readers
Attention Score in Context
| Chapter title |
Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments
|
|---|---|
| Chapter number | 15 |
| Book title |
Super-Resolution Microscopy
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7265-4_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7264-7, 978-1-4939-7265-4
|
| Authors |
Jonathan B. Grimm, Timothy A. Brown, Brian P. English, Timothée Lionnet, Luke D. Lavis |
| Abstract |
The development of genetically encoded self-labeling protein tags such as the HaloTag and SNAP-tag has expanded the utility of chemical dyes in microscopy. Intracellular labeling using these systems requires small, cell-permeable dyes with high brightness and photostability. We recently discovered a general method to improve the properties of classic fluorophores by replacing N,N-dimethylamino groups with four-membered azetidine rings to create the "Janelia Fluor" dyes. Here, we describe the synthesis of the HaloTag and SNAP-tag ligands of Janelia Fluor 549 and Janelia Fluor 646 as well as standard labeling protocols for use in ensemble and single-molecule cellular imaging. |
X Demographics
The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 71% |
| Portugal | 1 | 14% |
| Unknown | 1 | 14% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 4 | 57% |
| Members of the public | 3 | 43% |
Mendeley readers
The data shown below were compiled from readership statistics for 120 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 120 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 33 | 28% |
| Researcher | 18 | 15% |
| Student > Master | 16 | 13% |
| Student > Bachelor | 10 | 8% |
| Student > Doctoral Student | 5 | 4% |
| Other | 17 | 14% |
| Unknown | 21 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 45 | 38% |
| Agricultural and Biological Sciences | 12 | 10% |
| Neuroscience | 9 | 8% |
| Chemistry | 9 | 8% |
| Physics and Astronomy | 7 | 6% |
| Other | 14 | 12% |
| Unknown | 24 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 September 2017.
All research outputs
#7,696,749
of 25,387,480 outputs
Outputs from Methods in molecular biology
#2,281
of 14,198 outputs
Outputs of similar age
#129,705
of 428,476 outputs
Outputs of similar age from Methods in molecular biology
#236
of 1,087 outputs
Altmetric has tracked 25,387,480 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 14,198 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 428,476 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.
We're also able to compare this research output to 1,087 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.