Title |
The Use of Hyperimmune Chicken Reference Sera Is Not Appropriate for the Validation of Influenza Pseudotype Neutralization Assays
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Published in |
Pathogens, September 2017
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DOI | 10.3390/pathogens6040045 |
Pubmed ID | |
Authors |
Francesca Ferrara, Eleonora Molesti, Simon Scott, Giovanni Cattoli, Nigel Temperton |
Abstract |
The pseudotype particle neutralization test (pp-NT) is a next-generation serological assay employed for the sensitive study of influenza antibody responses against hemagglutinin (HA), including stalk-directed antibodies. However, a validation of this assay has yet to be performed, and this limits its use to primarily research laboratories. To identify possible serological standards to be used in optimization and validation of the pp-NT, we have evaluated the cross-reactivity of hyperimmune chicken reference antisera in this assay. Our findings show that the cross-reactivity detected by the pp-NT is only partly explained by phylogenetic relationships and protein homology between the HA subtypes analysed; further studies are necessary to understand the origin of the cross-reactivity detected, and reference standards with higher specificity should be evaluated or generated de novo for future use in pp-NT. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 33% |
United Kingdom | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Other | 1 | 14% |
Lecturer | 1 | 14% |
Student > Bachelor | 1 | 14% |
Student > Ph. D. Student | 1 | 14% |
Researcher | 1 | 14% |
Other | 0 | 0% |
Unknown | 2 | 29% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 2 | 29% |
Veterinary Science and Veterinary Medicine | 1 | 14% |
Agricultural and Biological Sciences | 1 | 14% |
Computer Science | 1 | 14% |
Unknown | 2 | 29% |