Title |
A single gp120 residue can affect HIV-1 tropism in macaques
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Published in |
PLoS Pathogens, September 2017
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DOI | 10.1371/journal.ppat.1006572 |
Pubmed ID | |
Authors |
Gregory Q. Del Prete, Brandon F. Keele, Jeannine Fode, Keyur Thumar, Adrienne E. Swanstrom, Anthony Rodriguez, Alice Raymond, Jacob D. Estes, Celia C. LaBranche, David C. Montefiori, Vineet N. KewalRamani, Jeffrey D. Lifson, Paul D. Bieniasz, Theodora Hatziioannou |
Abstract |
Species-dependent variation in proteins that aid or limit virus replication determines the ability of lentiviruses to jump between host species. Identifying and overcoming these differences facilitates the development of animal models for HIV-1, including models based on chimeric SIVs that express HIV-1 envelope (Env) glycoproteins, (SHIVs) and simian-tropic HIV-1 (stHIV) strains. Here, we demonstrate that the inherently poor ability of most HIV-1 Env proteins to use macaque CD4 as a receptor is improved during adaptation by virus passage in macaques. We identify a single amino acid, A281, in HIV-1 Env that consistently changes during adaptation in macaques and affects the ability of HIV-1 Env to use macaque CD4. Importantly, mutations at A281 do not markedly affect HIV-1 Env neutralization properties. Our findings should facilitate the design of HIV-1 Env proteins for use in non-human primate models and thus expedite the development of clinically relevant reagents for testing interventions against HIV-1. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 14 | 50% |
Australia | 2 | 7% |
United Kingdom | 2 | 7% |
Venezuela, Bolivarian Republic of | 1 | 4% |
Switzerland | 1 | 4% |
Unknown | 8 | 29% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 14 | 50% |
Scientists | 10 | 36% |
Science communicators (journalists, bloggers, editors) | 3 | 11% |
Practitioners (doctors, other healthcare professionals) | 1 | 4% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 22 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 27% |
Researcher | 4 | 18% |
Student > Bachelor | 2 | 9% |
Professor | 1 | 5% |
Lecturer | 1 | 5% |
Other | 2 | 9% |
Unknown | 6 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 6 | 27% |
Agricultural and Biological Sciences | 5 | 23% |
Biochemistry, Genetics and Molecular Biology | 5 | 23% |
Unknown | 6 | 27% |