Title |
Visible Multiphoton Dissociation of Chromophore-Tagged Peptides
|
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Published in |
Journal of the American Society for Mass Spectrometry, July 2017
|
DOI | 10.1007/s13361-017-1733-9 |
Pubmed ID | |
Authors |
Mathilde Bouakil, Alexander Kulesza, Steven Daly, Luke MacAleese, Rodolphe Antoine, Philippe Dugourd |
Abstract |
The visible photodissociation mechanisms of QSY7-tagged peptides of increasing size have been investigated by coupling a mass spectrometer and an optical parametric oscillator laser beam. The experiments herein consist of energy resolved collision- and laser-induced dissociation measurements on the chromophore-tagged peptides. The results show that fragmentation occurs by similar channels in both activation methods, but that the branching ratios are vastly different. Observation of a size-dependent minimum laser pulse energy required to induce fragmentation, and collisional cooling rates in time resolved experiments show that laser-induced dissociation occurs through the absorption of multiple photons by the chromophore and the subsequent heating through vibrational energy redistribution. The differences in branching ratio between collision- and laser-induced dissociation can then be understood by the highly anisotropic energy distribution following absorption of a photon. Graphical Abstract ᅟ. |
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