| Title |
Isolation and functional characterization of a methyl jasmonate-responsive 3-carene synthase from Lavandula x intermedia
|
|---|---|
| Published in |
Plant Molecular Biology, March 2017
|
| DOI | 10.1007/s11103-017-0588-6 |
| Pubmed ID | |
| Authors |
Ayelign M. Adal, Lukman S. Sarker, Ashley D. Lemke, Soheil S. Mahmoud |
| Abstract |
A methyl jasmonate responsive 3-carene synthase (Li3CARS) gene was isolated from Lavandula x intermedia and functionally characterized in vitro. Lavenders produce essential oils consisting mainly of monoterpenes, including the potent antimicrobial and insecticidal monoterpene 3-carene. In this study we isolated and functionally characterized a leaf-specific, methyl jasmonate (MeJA)-responsive monoterpene synthase (Li3CARS) from Lavandula x intermedia. The ORF excluding transit peptides encoded a 64.9 kDa protein that was expressed in E. coli, and purified with Ni-NTA agarose affinity chromatography. The recombinant Li3CARS converted GPP into 3-carene as the major product, with K m and k cat of 3.69 ± 1.17 µM and 2.01 s(-1) respectively. Li3CARS also accepted NPP as a substrate to produce multiple products including a small amount of 3-carene. The catalytic efficiency of Li3CARS to produce 3-carene was over ten fold higher for GPP (k cat /K m = 0.56 µM(-1)s(-1)) than NPP (k cat /K m = 0.044 µM(-1)s(-1)). Production of distinct end product profiles from different substrates (GPP versus NPP) by Li3CARS indicates that monoterpene metabolism may be controlled in part through substrate availability. Li3CARS transcripts were found to be highly abundant in leaves (16-fold) as compared to flower tissues. The transcriptional activity of Li3CARS correlated with 3-carene production, and was up-regulated (1.18- to 3.8-fold) with MeJA 8-72 h post-treatment. The results suggest that Li3CARS may have a defensive role in Lavandula. |
Mendeley readers
The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 31 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 19% |
| Student > Ph. D. Student | 5 | 16% |
| Student > Master | 4 | 13% |
| Researcher | 2 | 6% |
| Professor | 1 | 3% |
| Other | 3 | 10% |
| Unknown | 10 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 29% |
| Biochemistry, Genetics and Molecular Biology | 8 | 26% |
| Chemistry | 2 | 6% |
| Unspecified | 1 | 3% |
| Environmental Science | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 9 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 September 2017.
All research outputs
#4,220,894
of 23,003,906 outputs
Outputs from Plant Molecular Biology
#357
of 2,846 outputs
Outputs of similar age
#76,021
of 310,530 outputs
Outputs of similar age from Plant Molecular Biology
#3
of 19 outputs
Altmetric has tracked 23,003,906 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,846 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done well, scoring higher than 86% of its peers.
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We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.