| Title |
Molecular Characterization of Mosquitocidal Toxin (Surface Layer Protein, SLP) from Bacillus cereus VCRC B540
|
|---|---|
| Published in |
Applied Biochemistry and Biotechnology, September 2017
|
| DOI | 10.1007/s12010-017-2602-5 |
| Pubmed ID | |
| Authors |
Chinnasamy Mani, Jeyaperumal Selvakumari, YeonSoo Han, YongHun Jo, Krishnaraj Thirugnanasambantham, Somaiah Sundarapandian, Subbiah Poopathi |
| Abstract |
A marine Bacillus cereus (VCRC B540) with mosquitocidal effect was recently reported from red snapper fish (Lutjanus sanguineous) gut and surface layer protein (S-layer protein, SLP) was reported to be mosquito larvicidal factor. In this present study, the gene encoding the surface layer protein was amplified from the genomic DNA and functionally characterized. Amplification of SLP-encoding gene revealed 1,518 bp PCR product, and analysis of the sequence revealed the presence of 1482 bp open reading frame with coding capacity for a polypeptide of 493 amino acids. Phylogenetic analysis revealed with homology among closely related Bacillus cereus groups of organisms as well as Bacillus strains. Removal of nucleotides encoding signaling peptide revealed the functional cloning fragment of length 1398 bp. Theoretical molecular weight (51.7 kDa) and isoelectric point (5.99) of the deduced functional SLP protein were predicted using ProtParam. The amplified PCR product was cloned into a plasmid vector (pGEM-T), and the open reading frame free off signaling peptide was subsequently cloned inpET-28a(+) and expressed in Escherichia coli BL21 (DE3). The isopropyl-β-D-thiogalactopyranoside (IPTG)-induced recombinant SLP was confirmed using western blotting, and functional SLP revealed mosquito larvicidal property. Therefore, the major findings revealed that SLP is a factor responsible for mosquitocidal activity, and the molecular characterization of this toxin was extensively studied. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 13% |
| Student > Master | 2 | 13% |
| Student > Ph. D. Student | 2 | 13% |
| Unspecified | 1 | 6% |
| Other | 1 | 6% |
| Other | 2 | 13% |
| Unknown | 6 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 13% |
| Medicine and Dentistry | 2 | 13% |
| Business, Management and Accounting | 1 | 6% |
| Unspecified | 1 | 6% |
| Physics and Astronomy | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 8 | 50% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 May 2022.
All research outputs
#6,487,734
of 23,003,906 outputs
Outputs from Applied Biochemistry and Biotechnology
#471
of 2,527 outputs
Outputs of similar age
#105,058
of 320,414 outputs
Outputs of similar age from Applied Biochemistry and Biotechnology
#4
of 41 outputs
Altmetric has tracked 23,003,906 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 2,527 research outputs from this source. They receive a mean Attention Score of 3.2. This one has done well, scoring higher than 80% of its peers.
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We're also able to compare this research output to 41 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.