| Title |
The environment shapes microbial enzymes: five cold-active and salt-resistant carboxylesterases from marine metagenomes
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, September 2014
|
| DOI | 10.1007/s00253-014-6038-3 |
| Pubmed ID | |
| Authors |
Anatoli Tchigvintsev, Hai Tran, Ana Popovic, Filip Kovacic, Greg Brown, Robert Flick, Mahbod Hajighasemi, Olga Egorova, Joseph C. Somody, Dmitri Tchigvintsev, Anna Khusnutdinova, Tatyana N. Chernikova, Olga V. Golyshina, Michail M. Yakimov, Alexei Savchenko, Peter N. Golyshin, Karl-Erich Jaeger, Alexander F. Yakunin |
| Abstract |
Most of the Earth's biosphere is cold and is populated by cold-adapted microorganisms. To explore the natural enzyme diversity of these environments and identify new carboxylesterases, we have screened three marine metagenome gene libraries for esterase activity. The screens identified 23 unique active clones, from which five highly active esterases were selected for biochemical characterization. The purified metagenomic esterases exhibited high activity against α-naphthyl and p-nitrophenyl esters with different chain lengths. All five esterases retained high activity at 5 °C indicating that they are cold-adapted enzymes. The activity of MGS0010 increased more than two times in the presence of up to 3.5 M NaCl or KCl, whereas the other four metagenomic esterases were inhibited to various degrees by these salts. The purified enzymes showed different sensitivities to inhibition by solvents and detergents, and the activities of MGS0010, MGS0105 and MGS0109 were stimulated three to five times by the addition of glycerol. Screening of purified esterases against 89 monoester substrates revealed broad substrate profiles with a preference for different esters. The metagenomic esterases also hydrolyzed several polyester substrates including polylactic acid suggesting that they can be used for polyester depolymerization. Thus, esterases from marine metagenomes are cold-adapted enzymes exhibiting broad biochemical diversity reflecting the environmental conditions where they evolved. |
Mendeley readers
The data shown below were compiled from readership statistics for 134 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Russia | 1 | <1% |
| Unknown | 133 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 24 | 18% |
| Student > Master | 19 | 14% |
| Researcher | 15 | 11% |
| Student > Bachelor | 13 | 10% |
| Student > Doctoral Student | 9 | 7% |
| Other | 20 | 15% |
| Unknown | 34 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 35 | 26% |
| Agricultural and Biological Sciences | 32 | 24% |
| Environmental Science | 9 | 7% |
| Chemistry | 6 | 4% |
| Immunology and Microbiology | 4 | 3% |
| Other | 7 | 5% |
| Unknown | 41 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 September 2014.
All research outputs
#16,371,088
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#5,817
of 8,034 outputs
Outputs of similar age
#142,367
of 242,982 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#48
of 102 outputs
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