| Title |
Imaging Mass Spectrometry of a Coral Microbe Interaction with Fungi
|
|---|---|
| Published in |
Journal of Chemical Ecology, July 2013
|
| DOI | 10.1007/s10886-013-0320-1 |
| Pubmed ID | |
| Authors |
Wilna J. Moree, Jane Y. Yang, Xiling Zhao, Wei-Ting Liu, Marystella Aparicio, Librada Atencio, Javier Ballesteros, Joel Sánchez, Ronnie G. Gavilán, Marcelino Gutiérrez, Pieter C. Dorrestein |
| Abstract |
Fungal infections are increasing worldwide, including in the aquatic environment. Microbiota that coexist with marine life can provide protection against fungal infections by secretion of metabolites with antifungal properties. Our laboratory has developed mass spectrometric methodologies with the goal of improving our functional understanding of microbial metabolites and guiding the discovery process of anti-infective agents from natural sources. GA40, a Bacillus amyloliquefaciens strain isolated from an octocoral in Panama, displayed antifungal activity against various terrestrial and marine fungal strains. Using matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS), the molecular species produced by this microbe were visualized in a side-by-side interaction with two representative fungal strains, Aspergillus fumigatus and Aspergillus niger. The visualization was performed directly on the agar without the need for extraction. By evaluating the spatial distributions, relative intensities and m/z values of GA40 secreted metabolites in the fungal interactions and singly grown control colonies, we obtained insight into the antifungal activity of secreted metabolites. Annotation of GA40 metabolites observed in MALDI-IMS was facilitated by MS/MS networking analysis, a mass spectrometric technique that clusters metabolites with similar MS/MS fragmentation patterns. This analysis established that the predominant GA40 metabolites belong to the iturin family. In a fungal inhibition assay of A. fumigatus, the GA40 iturin metabolites were found to be responsible for the antifungal properties of this Bacillus strain. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 130 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 3 | 2% |
| Colombia | 1 | <1% |
| Switzerland | 1 | <1% |
| Germany | 1 | <1% |
| Kazakhstan | 1 | <1% |
| Panama | 1 | <1% |
| Unknown | 122 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 34 | 26% |
| Researcher | 26 | 20% |
| Student > Master | 14 | 11% |
| Student > Doctoral Student | 12 | 9% |
| Student > Bachelor | 9 | 7% |
| Other | 18 | 14% |
| Unknown | 17 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 40 | 31% |
| Chemistry | 28 | 22% |
| Biochemistry, Genetics and Molecular Biology | 15 | 12% |
| Immunology and Microbiology | 10 | 8% |
| Environmental Science | 6 | 5% |
| Other | 13 | 10% |
| Unknown | 18 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 September 2014.
All research outputs
#18,378,085
of 22,763,032 outputs
Outputs from Journal of Chemical Ecology
#1,749
of 2,048 outputs
Outputs of similar age
#148,409
of 198,032 outputs
Outputs of similar age from Journal of Chemical Ecology
#14
of 20 outputs
Altmetric has tracked 22,763,032 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,048 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.1. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 198,032 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one is in the 5th percentile – i.e., 5% of its contemporaries scored the same or lower than it.