| Title |
Expression and characterization of a new heat-stable endo-type alginate lyase from deep-sea bacterium Flammeovirga sp. NJ-04
|
|---|---|
| Published in |
Extremophiles, September 2017
|
| DOI | 10.1007/s00792-017-0962-y |
| Pubmed ID | |
| Authors |
Benwei Zhu, Fang Ni, Yun Sun, Zhong Yao |
| Abstract |
Alginate lyases play an essential role in the production of oligosaccharides by degrading alginate polysaccharide. Although many alginate lyases from various microorganisms have been characterized, reports on alginate lyases with special characteristics and commercial potential are still rather rare. In this study, a new alginate lyase, FsAlgA, was cloned from the deep-sea marine bacterium Flammeovirga sp. NJ-04. The recombinant enzyme was purified on Ni-NTA sepharose and then characterized in detail. It exhibited the highest activity (3343.7 U/mg) at pH 7.0 and 50 °C. Notably, the FsAlgA retained more than 80% of its maximum activity after incubation at 50 °C for 30 min, suggesting that FsAlgA was a heat-stable alginate lyase. Additionally, FsAlgA possessed broad substrate specificity, showing high activities toward both poly β-D-mannuronate (polyM) and poly α-L-guluronate (polyG). Furthermore, the K m values of FsAlgA toward sodium alginate (0.48 mM) and polyG (0.94 mM) were lower than that toward polyM (1.42 mM). The TLC and ESI-MS analyses indicated that FsAlgA endolytically degraded alginate polysaccharide and released oligosaccharides with degree of polymerization (DP) of 2-5. Therefore, it may be a potent tool to produce alginate oligosaccharides with low DPs. |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 17% |
| Unspecified | 2 | 11% |
| Student > Doctoral Student | 2 | 11% |
| Professor | 1 | 6% |
| Student > Bachelor | 1 | 6% |
| Other | 2 | 11% |
| Unknown | 7 | 39% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 28% |
| Unspecified | 2 | 11% |
| Agricultural and Biological Sciences | 2 | 11% |
| Earth and Planetary Sciences | 1 | 6% |
| Immunology and Microbiology | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 7 | 39% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 October 2021.
All research outputs
#3,775,422
of 23,003,906 outputs
Outputs from Extremophiles
#92
of 801 outputs
Outputs of similar age
#68,170
of 319,601 outputs
Outputs of similar age from Extremophiles
#6
of 18 outputs
Altmetric has tracked 23,003,906 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 801 research outputs from this source. They receive a mean Attention Score of 4.2. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 319,601 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 78% of its contemporaries.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.