| Title |
Preanalytical variables and performance of diagnostic RNA-based gene expression analysis in breast cancer
|
|---|---|
| Published in |
Virchows Archiv, September 2014
|
| DOI | 10.1007/s00428-014-1652-0 |
| Pubmed ID | |
| Authors |
Christopher Poremba, Jennifer Uhlendorff, Berit M. Pfitzner, Guido Hennig, Kerstin Bohmann, Hans Bojar, Veit Krenn, Jan C. Brase, Franziska Haufe, Manuela Averdick, Manfred Dietel, Ralf Kronenwett, Carsten Denkert |
| Abstract |
Prognostic multigene expression assays have become widely available to provide additional information to standard clinical parameters and to support clinicians in treatment decisions. In this study, we analyzed the impact of variations in tissue handling on the diagnostic EndoPredict test results. EndoPredict is a quantitative reverse transcription PCR assay conducted on RNA from formalin-fixed, paraffin-embedded (FFPE) tissue that predicts the likelihood of distant recurrence in patients with ER-positive/HER2-negative breast cancer. In this study, we performed a total of 138 EndoPredict assays to study the effects of preanalytical variables such as time to fixation, fixation time, tumor cell content, and section storage time on the EndoPredict test results. A time to fixation of up to 12 h and fixation of up to 5 days did not affect the results of the gene expression test. Paired samples of FFPE sections with tumor cell content ranging from 15 to 95 % and tumor-enriched samples showed a correlation coefficient of 0.97. Test results of tissue sections that have been stored for 12 months at +4 or +20 °C showed a correlation of 0.99 when compared to results of nonstored sections. In conclusion, preanalytical tissue handling is not a critical factor for diagnostic gene expression analysis with the EndoPredict assay. The test can therefore be easily integrated into the standard workflow of molecular pathology. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Denmark | 1 | 3% |
| Brazil | 1 | 3% |
| Unknown | 38 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 20% |
| Student > Bachelor | 8 | 20% |
| Student > Ph. D. Student | 4 | 10% |
| Other | 3 | 8% |
| Student > Doctoral Student | 2 | 5% |
| Other | 6 | 15% |
| Unknown | 9 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 23% |
| Medicine and Dentistry | 9 | 23% |
| Engineering | 3 | 8% |
| Computer Science | 2 | 5% |
| Biochemistry, Genetics and Molecular Biology | 1 | 3% |
| Other | 5 | 13% |
| Unknown | 11 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 November 2016.
All research outputs
#6,091,294
of 22,763,032 outputs
Outputs from Virchows Archiv
#280
of 1,945 outputs
Outputs of similar age
#62,083
of 245,954 outputs
Outputs of similar age from Virchows Archiv
#2
of 18 outputs
Altmetric has tracked 22,763,032 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 1,945 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done well, scoring higher than 85% of its peers.
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We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.