Title |
Gene structure, regulatory control, and evolution of black widow venom latrotoxins
|
---|---|
Published in |
Febs Letters, September 2014
|
DOI | 10.1016/j.febslet.2014.08.034 |
Pubmed ID | |
Authors |
Kanaka Varun Bhere, Robert A. Haney, Nadia A. Ayoub, Jessica E. Garb |
Abstract |
Black widow venom contains α-latrotoxin, infamous for causing intense pain. Combining 33kb of Latrodectus hesperus genomic DNA with RNA-Seq, we characterized the α-latrotoxin gene and discovered a paralog, 4.5kb downstream. Both paralogs exhibit venom gland specific transcription, and may be regulated post-transcriptionally via musashi-like proteins. A 4kb intron interrupts the α-latrotoxin coding sequence, while a 10kb intron in the 3' UTR of the paralog may cause non-sense-mediated decay. Phylogenetic analysis confirms these divergent latrotoxins diversified through recent tandem gene duplications. Thus, latrotoxin genes have more complex structures, regulatory controls, and sequence diversity than previously proposed. |
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Unknown | 4 | 67% |
Demographic breakdown
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Scientists | 2 | 33% |
Mendeley readers
Geographical breakdown
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Researcher | 7 | 18% |
Student > Ph. D. Student | 4 | 10% |
Student > Postgraduate | 3 | 8% |
Student > Master | 3 | 8% |
Other | 6 | 15% |
Unknown | 4 | 10% |
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Neuroscience | 2 | 5% |
Other | 6 | 15% |
Unknown | 5 | 13% |