| Title |
RETRACTED ARTICLE: BMP2-Smad-Mediated SH-SY5Y Neuroblastoma Cell Proliferation and Neurite Outgrowth Are Regulated Through Dynamin-Dependent Endocytosis
|
|---|---|
| Published in |
Molecular Neurobiology, September 2014
|
| DOI | 10.1007/s12035-014-8896-1 |
| Pubmed ID | |
| Authors |
Xiangshan Yang, Shunzeng Lv, Daotang Li, Wenyuan Lv, Kaixi Fan, Lijun Sheng, Ranran Shi, Jing Zhang, Man Feng, Zhongfa Xu |
| Abstract |
Smad signalling plays an important role in the neurobiology, which can be induced by bone morphogenetic protein 2 (BMP2) and be regulated by endocytosis. However, it is still unclear whether endocytosis regulates BMP2-Smad-mediated proliferation and neurite growth in SH-SY5Y. Here we investigated the effects of endocytosis on BMP2-mediated Smad signallings using the neuroblastoma SH-SY5Y cell. In this study, using dynasore, dynamin-dependent endocytosis was assayed; using Western blot and neurite outgrowth assay, BMP2-Smad signalling and SH-SY5Y neurite outgrowth were measured. In the present study, our data showed that dynasore indeed inhibited dynamin-dependent endocytosis in SH-SY5Y. The Smad 1/5/8 phosphorylation level was upregulated in response to exogenous BMP2, while BMP2-induced p-Smad 1/5/8 expression was significantly suppressed due to BMP2 and dynasore co-treatment (p < 0.001). In addition, the immunocytochemical staining revealed high nuclear expression of p-Smad 1/5/8 in response to BMP2 induction, whereas BMP2 and dynasore reduced BMP2-induced p-Smad 1/5/8 expression (p < 0.001). Besides, BMP2-induced SH-SY5Y proliferation and neurite outgrowth were effectively affected by the inhibition of dynamin-dependent endocytosis. In conclusion, dynamin-dependent endocytosis regulates BMP2-Smad signalling in the SH-SY5Y cells and further interferes with the proliferation and neurite outgrowth of SH-SH5Y, which offers a new therapeutic prospect for the neuroblastoma. |
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 33% |
| United Kingdom | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 63% |
| Lecturer | 1 | 13% |
| Student > Doctoral Student | 1 | 13% |
| Student > Master | 1 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 38% |
| Biochemistry, Genetics and Molecular Biology | 1 | 13% |
| Linguistics | 1 | 13% |
| Immunology and Microbiology | 1 | 13% |
| Medicine and Dentistry | 1 | 13% |
| Other | 1 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 June 2017.
All research outputs
#3,680,054
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Outputs from Molecular Neurobiology
#805
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Outputs of similar age
#40,060
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Outputs of similar age from Molecular Neurobiology
#4
of 53 outputs
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