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Identification of Electric-Field-Dependent Steps in the Na+,K+-Pump Cycle

Overview of attention for article published in Biophysical Journal, September 2014
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Title
Identification of Electric-Field-Dependent Steps in the Na+,K+-Pump Cycle
Published in
Biophysical Journal, September 2014
DOI 10.1016/j.bpj.2014.05.054
Pubmed ID
Authors

Laura J. Mares, Alvaro Garcia, Helge H. Rasmussen, Flemming Cornelius, Yasser A. Mahmmoud, Joshua R. Berlin, Bogdan Lev, Toby W. Allen, Ronald J. Clarke

Abstract

The charge-transporting activity of the Na(+),K(+)-ATPase depends on its surrounding electric field. To isolate which steps of the enzyme's reaction cycle involve charge movement, we have investigated the response of the voltage-sensitive fluorescent probe RH421 to interaction of the protein with BTEA (benzyltriethylammonium), which binds from the extracellular medium to the Na(+),K(+)-ATPase's transport sites in competition with Na(+) and K(+), but is not occluded within the protein. We find that only the occludable ions Na(+), K(+), Rb(+), and Cs(+) cause a drop in RH421 fluorescence. We conclude that RH421 detects intramembrane electric field strength changes arising from charge transport associated with conformational changes occluding the transported ions within the protein, not the electric fields of the bound ions themselves. This appears at first to conflict with electrophysiological studies suggesting extracellular Na(+) or K(+) binding in a high field access channel is a major electrogenic reaction of the Na(+),K(+)-ATPase. All results can be explained consistently if ion occlusion involves local deformations in the lipid membrane surrounding the protein occurring simultaneously with conformational changes necessary for ion occlusion. The most likely origin of the RH421 fluorescence response is a change in membrane dipole potential caused by membrane deformation.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 4%
Unknown 24 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 44%
Student > Bachelor 3 12%
Student > Ph. D. Student 3 12%
Student > Master 2 8%
Lecturer > Senior Lecturer 1 4%
Other 2 8%
Unknown 3 12%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 32%
Agricultural and Biological Sciences 6 24%
Chemistry 3 12%
Physics and Astronomy 2 8%
Pharmacology, Toxicology and Pharmaceutical Science 1 4%
Other 2 8%
Unknown 3 12%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 September 2014.
All research outputs
#17,285,036
of 25,373,627 outputs
Outputs from Biophysical Journal
#7,361
of 10,295 outputs
Outputs of similar age
#148,727
of 248,666 outputs
Outputs of similar age from Biophysical Journal
#54
of 99 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 10,295 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.3. This one is in the 19th percentile – i.e., 19% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 248,666 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 99 others from the same source and published within six weeks on either side of this one. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.