| Title |
Nile Red staining of phytoplankton neutral lipids: species-specific fluorescence kinetics in various solvents
|
|---|---|
| Published in |
Journal of Applied Phycology, September 2014
|
| DOI | 10.1007/s10811-014-0404-5 |
| Pubmed ID | |
| Authors |
Katariina Natunen, Jukka Seppälä, Dagmar Schwenk, Heiko Rischer, Kristian Spilling, Timo Tamminen |
| Abstract |
Nile Red (NR) staining potentially offers a simple method for monitoring lipid accumulation in microalgal cultivation. However, variable staining efficiencies and methods have been reported. The effect of dimethyl sulfoxide (DMSO), ethylene glycol (EG) and glycerol on NR penetration with four different phytoplankton species representing different taxonomical groups was studied. Treatment with the solvents enhanced the NR fluorescence of the diatom Phaeodactylum tricornutum during kinetic fluorescence measurements, but high concentrations of solvents were needed. None of the solvents improved NR staining of the green alga Chlorella pyrenoidosa and Scenedesmus obliquus, which are known to be difficult to stain due to their thick and rigid cell walls. The naked Isochrysis sp. cells stained best without solvents. The results confirm that NR staining protocol needs to be optimized for each species. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 68 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Belgium | 1 | 1% |
| Unknown | 67 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 22% |
| Student > Master | 12 | 18% |
| Researcher | 7 | 10% |
| Professor > Associate Professor | 5 | 7% |
| Student > Bachelor | 3 | 4% |
| Other | 13 | 19% |
| Unknown | 13 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 21 | 31% |
| Biochemistry, Genetics and Molecular Biology | 11 | 16% |
| Environmental Science | 7 | 10% |
| Engineering | 5 | 7% |
| Chemistry | 3 | 4% |
| Other | 5 | 7% |
| Unknown | 16 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 July 2023.
All research outputs
#7,780,161
of 24,169,085 outputs
Outputs from Journal of Applied Phycology
#448
of 2,613 outputs
Outputs of similar age
#79,247
of 253,967 outputs
Outputs of similar age from Journal of Applied Phycology
#8
of 64 outputs
Altmetric has tracked 24,169,085 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 2,613 research outputs from this source. They receive a mean Attention Score of 3.2. This one has done well, scoring higher than 79% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 253,967 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 64 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.