Title |
Protocol: a fast and simple in situ PCR method for localising gene expression in plant tissue
|
---|---|
Published in |
Plant Methods, September 2014
|
DOI | 10.1186/1746-4811-10-29 |
Pubmed ID | |
Authors |
Asmini Athman, Sandra K Tanz, Vanessa M Conn, Charlotte Jordans, Gwenda M Mayo, Weng W Ng, Rachel A Burton, Simon J Conn, Matthew Gilliham |
Abstract |
An important step in characterising the function of a gene is identifying the cells in which it is expressed. Traditional methods to determine this include in situ hybridisation, gene promoter-reporter fusions or cell isolation/purification techniques followed by quantitative PCR. These methods, although frequently used, can have limitations including their time-consuming nature, limited specificity, reliance upon well-annotated promoters, high cost, and the need for specialized equipment. In situ PCR is a relatively simple and rapid method that involves the amplification of specific mRNA directly within plant tissue whilst incorporating labelled nucleotides that are subsequently detected by immunohistochemistry. Another notable advantage of this technique is that it can be used on plants that are not easily genetically transformed. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 3 | 38% |
Australia | 3 | 38% |
Unknown | 2 | 25% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 4 | 50% |
Scientists | 3 | 38% |
Science communicators (journalists, bloggers, editors) | 1 | 13% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 2 | 1% |
Switzerland | 1 | <1% |
Chile | 1 | <1% |
Norway | 1 | <1% |
Brazil | 1 | <1% |
Sweden | 1 | <1% |
India | 1 | <1% |
Argentina | 1 | <1% |
Spain | 1 | <1% |
Other | 0 | 0% |
Unknown | 167 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 41 | 23% |
Student > Ph. D. Student | 28 | 16% |
Student > Master | 23 | 13% |
Student > Bachelor | 17 | 10% |
Student > Doctoral Student | 12 | 7% |
Other | 26 | 15% |
Unknown | 30 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 97 | 55% |
Biochemistry, Genetics and Molecular Biology | 30 | 17% |
Environmental Science | 4 | 2% |
Immunology and Microbiology | 2 | 1% |
Medicine and Dentistry | 2 | 1% |
Other | 9 | 5% |
Unknown | 33 | 19% |