Title |
Role of Alanine Racemase Mutations in Mycobacterium tuberculosis d-Cycloserine Resistance
|
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Published in |
Antimicrobial Agents and Chemotherapy, November 2017
|
DOI | 10.1128/aac.01575-17 |
Pubmed ID | |
Authors |
Yoshio Nakatani, Helen K. Opel-Reading, Matthias Merker, Diana Machado, Sönke Andres, S. Siva Kumar, Danesh Moradigaravand, Francesc Coll, João Perdigão, Isabel Portugal, Thomas Schön, Dina Nair, K. R. Uma Devi, Thomas A. Kohl, Patrick Beckert, Taane G. Clark, Gugu Maphalala, Derrick Khumalo, Roland Diel, Kadri Klaos, Htin Lin Aung, Gregory M. Cook, Julian Parkhill, Sharon J. Peacock, Soumya Swaminathan, Miguel Viveiros, Stefan Niemann, Kurt L. Krause, Claudio U. Köser |
Abstract |
Screening of more than 1,500 drug-resistant strains of Mycobacterium tuberculosis revealed evolutionary patterns characteristic of positive selection for three alanine racemase (Alr) mutations. We investigated these mutations using molecular modeling, in vitro MIC testing, as well as direct measurements of enzymatic activity, which demonstrated that these mutations likely confer resistance to D-cycloserine. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 20% |
Unknown | 4 | 80% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 4 | 80% |
Scientists | 1 | 20% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 74 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 14 | 19% |
Student > Master | 12 | 16% |
Professor | 6 | 8% |
Student > Bachelor | 5 | 7% |
Student > Ph. D. Student | 4 | 5% |
Other | 10 | 14% |
Unknown | 23 | 31% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 17 | 23% |
Immunology and Microbiology | 7 | 9% |
Agricultural and Biological Sciences | 6 | 8% |
Medicine and Dentistry | 6 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 5 | 7% |
Other | 6 | 8% |
Unknown | 27 | 36% |