| Title |
Bottom-Up Two-Dimensional Electron-Capture Dissociation Mass Spectrometry of Calmodulin
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, October 2017
|
| DOI | 10.1007/s13361-017-1812-y |
| Pubmed ID | |
| Authors |
Federico Floris, Maria A. van Agthoven, Lionel Chiron, Christopher A. Wootton, Pui Yiu Yuko Lam, Mark P. Barrow, Marc-André Delsuc, Peter B. O’Connor |
| Abstract |
Two-dimensional mass spectrometry (2D MS) is a tandem mass spectrometry technique that allows data-independent fragmentation of all precursors in a mixture without previous isolation, through modulation of the ion cyclotron frequency in the ICR-cell prior to fragmentation. Its power as an analytical technique has been proven particularly for proteomics. Recently, a comparison study between 1D and 2D MS has been performed using infrared multiphoton dissociation (IRMPD) on calmodulin (CaM), highlighting the capabilities of the technique in both top-down (TDP) and bottom-up proteomics (BUP). The goal of this work is to expand this study on CaM using electron-capture dissociation (ECD) 2D MS as a single complementary BUP experiment in order to enhance the cleavage coverage of the protein under analysis. By adding the results of the BUP 2D ECD MS to the 2D IRMPD MS analysis of CaM, the total cleavage coverage increased from ~40% to ~68%. Graphical abstract ᅟ. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 17% |
| United States | 1 | 17% |
| Canada | 1 | 17% |
| France | 1 | 17% |
| Unknown | 2 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 3 | 50% |
| Members of the public | 2 | 33% |
| Science communicators (journalists, bloggers, editors) | 1 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 25% |
| Unspecified | 2 | 10% |
| Student > Doctoral Student | 2 | 10% |
| Student > Bachelor | 2 | 10% |
| Lecturer > Senior Lecturer | 1 | 5% |
| Other | 6 | 30% |
| Unknown | 2 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 10 | 50% |
| Biochemistry, Genetics and Molecular Biology | 2 | 10% |
| Unspecified | 2 | 10% |
| Agricultural and Biological Sciences | 2 | 10% |
| Medicine and Dentistry | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 3 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 December 2017.
All research outputs
#7,716,445
of 25,382,440 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#1,053
of 3,835 outputs
Outputs of similar age
#114,126
of 331,602 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#12
of 62 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 3,835 research outputs from this source. They receive a mean Attention Score of 3.8. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 331,602 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 62 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.