| Title |
Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells
|
|---|---|
| Published in |
Stem Cell Reviews and Reports, December 2012
|
| DOI | 10.1007/s12015-012-9420-5 |
| Pubmed ID | |
| Authors |
Yimei Wang, Jinyu Liu, Xiaohua Tan, Gaofeng Li, Yunhe Gao, Xuejuan Liu, Lihong Zhang, Yulin Li |
| Abstract |
Reprogramming of somatic cells into inducible pluripotent stem cells (iPSCs) provides an alternative to using embryonic stem cells (ESCs). Mesenchymal stem cells derived from human hair follicles (hHF-MSCs) are easily accessible, reproducible by direct plucking of human hairs. Whether these hHF-MSCs can be reprogrammed has not been previously reported. Here we report the generation of iPSCs from hHF-MSCs obtained by plucking several hairs. hHF-MSCs were isolated from hair follicle tissues and their mesenchymal nature confirmed by detecting cell surface antigens and multilineage differentiation potential towards adipocytes and osteoblasts. They were then reprogrammed into iPSCs by lentiviral transduction with Oct4, Sox2, c-Myc and Klf4. hHF-MSC-derived iPSCs appeared indistinguishable from human embryonic stem cells (hESCs) in colony morphology, expression of alkaline phosphotase, and expression of specific hESCs surface markers, SSEA-3, SSEA-4, Tra-1-60, Tra-1-81, Nanog, Oct4, E-Cadherin and endogenous pluripotent genes. When injected into immunocompromised mice, hHF-MSC-derived iPSCs formed teratomas containing representatives of all three germ layers. This is the first study to report reprogramming of hHF-MSCs into iPSCs. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 77 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 1% |
| Spain | 1 | 1% |
| France | 1 | 1% |
| Canada | 1 | 1% |
| Unknown | 73 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 20 | 26% |
| Researcher | 12 | 16% |
| Student > Master | 12 | 16% |
| Student > Bachelor | 7 | 9% |
| Student > Doctoral Student | 7 | 9% |
| Other | 12 | 16% |
| Unknown | 7 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 30 | 39% |
| Biochemistry, Genetics and Molecular Biology | 15 | 19% |
| Medicine and Dentistry | 9 | 12% |
| Engineering | 5 | 6% |
| Neuroscience | 3 | 4% |
| Other | 8 | 10% |
| Unknown | 7 | 9% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 December 2016.
All research outputs
#3,622,206
of 25,374,647 outputs
Outputs from Stem Cell Reviews and Reports
#105
of 1,036 outputs
Outputs of similar age
#34,737
of 287,210 outputs
Outputs of similar age from Stem Cell Reviews and Reports
#2
of 5 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,036 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 287,210 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.