| Title |
Dynamic metabolic reprogramming of steroidal glycol-alkaloid and phenylpropanoid biosynthesis may impart early blight resistance in wild tomato (Solanum arcanum Peralta)
|
|---|---|
| Published in |
Plant Molecular Biology, October 2017
|
| DOI | 10.1007/s11103-017-0660-2 |
| Pubmed ID | |
| Authors |
Balkrishna A. Shinde, Bhushan B. Dholakia, Khalid Hussain, Sayantan Panda, Sagit Meir, Ilana Rogachev, Asaph Aharoni, Ashok P. Giri, Avinash C. Kamble |
| Abstract |
Exploration with high throughput leaf metabolomics along with functional genomics in wild tomato unreveal potential role of steroidal glyco-alkaloids and phenylpropanoids during early blight resistance. Alternaria solani severely affects tomato (Solanum lycopersicum L.) yield causing early blight (EB) disease in tropical environment. Wild relative, Solanum arcanum Peralta could be a potential source of EB resistance; however, its underlying molecular mechanism largely remains unexplored. Hence, non-targeted metabolomics was applied on resistant and susceptible S. arcanum accessions upon A. solani inoculation to unravel metabolic dynamics during different stages of disease progression. Total 2047 potential metabolite peaks (mass signals) were detected of which 681 and 684 metabolites revealed significant modulation and clear differentiation in resistant and susceptible accessions, respectively. Majority of the EB-triggered metabolic changes were active from steroidal glycol-alkaloid (SGA), lignin and flavonoid biosynthetic pathways. Further, biochemical and gene expression analyses of key enzymes from these pathways positively correlated with phenotypic variation in the S. arcanum accessions indicating their potential role in EB. Additionally, transcription factors regulating lignin biosynthesis were also up-regulated in resistant plants and electrophoretic mobility shift assay revealed sequence-specific binding of rSaWRKY1 with MYB20 promoter. Moreover, transcript accumulation of key genes from phenylpropanoid and SGA pathways along with WRKY and MYB in WRKY1 transgenic tomato lines supported above findings. Overall, this study highlights vital roles of SGAs as phytoalexins and phenylpropanoids along with lignin accumulation unrevealing possible mechanistic basis of EB resistance in wild tomato. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 57 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 23% |
| Researcher | 8 | 14% |
| Student > Master | 7 | 12% |
| Student > Postgraduate | 3 | 5% |
| Student > Bachelor | 3 | 5% |
| Other | 6 | 11% |
| Unknown | 17 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 24 | 42% |
| Biochemistry, Genetics and Molecular Biology | 5 | 9% |
| Chemistry | 2 | 4% |
| Nursing and Health Professions | 1 | 2% |
| Immunology and Microbiology | 1 | 2% |
| Other | 3 | 5% |
| Unknown | 21 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 November 2017.
All research outputs
#2,908,577
of 23,005,189 outputs
Outputs from Plant Molecular Biology
#111
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Outputs of similar age
#57,173
of 323,110 outputs
Outputs of similar age from Plant Molecular Biology
#1
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