| Title |
The nano-bio interface mapped by oxidative footprinting of the adsorption sites of myoglobin
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, September 2014
|
| DOI | 10.1007/s00216-014-8188-7 |
| Pubmed ID | |
| Authors |
Stéphanie Devineau, Christelle Mathé, Véronique Legros, Florence Gonnet, Régis Daniel, Jean Philippe Renault, Serge Pin |
| Abstract |
Oxidative footprinting has been used to study the structure of macromolecular assemblies such as protein-protein and protein-ligand complexes. We propose a novel development of this technique to probe the protein corona that forms at the surface of nanoparticles in any biological medium. Indeed, very few techniques allow studying this interface at the molecular and residue level. Based on hydroxyl radical-mediated oxidation of proteins and analysis by nanoscale liquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS), two sites of adsorption of myoglobin on silica nanoparticles are identified. This method gives new insights in the understanding of protein adsorption on nanomaterials. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 10% |
| Spain | 1 | 10% |
| Unknown | 8 | 80% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 50% |
| Student > Ph. D. Student | 3 | 30% |
| Lecturer | 1 | 10% |
| Student > Doctoral Student | 1 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 3 | 30% |
| Biochemistry, Genetics and Molecular Biology | 3 | 30% |
| Agricultural and Biological Sciences | 2 | 20% |
| Immunology and Microbiology | 1 | 10% |
| Veterinary Science and Veterinary Medicine | 1 | 10% |
| Other | 0 | 0% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 September 2014.
All research outputs
#20,655,488
of 25,371,288 outputs
Outputs from Analytical & Bioanalytical Chemistry
#6,601
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Outputs of similar age
#192,390
of 263,027 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#56
of 77 outputs
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