Title |
Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II
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Published in |
Journal of Cell Biology, September 2014
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DOI | 10.1083/jcb.201406033 |
Pubmed ID | |
Authors |
Deepak Adhikari, M. Kasim Diril, Kiran Busayavalasa, Sanjiv Risal, Shoma Nakagawa, Rebecca Lindkvist, Yan Shen, Vincenzo Coppola, Lino Tessarollo, Nobuaki R. Kudo, Philipp Kaldis, Kui Liu |
Abstract |
In mitosis, the Greatwall kinase (called microtubule-associated serine/threonine kinase like [Mastl] in mammals) is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity that is needed for the entry into MII in mouse oocytes. |
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France | 1 | 33% |
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Mendeley readers
Geographical breakdown
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Unknown | 62 | 97% |
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Student > Ph. D. Student | 17 | 27% |
Student > Master | 6 | 9% |
Student > Bachelor | 4 | 6% |
Student > Doctoral Student | 3 | 5% |
Other | 4 | 6% |
Unknown | 11 | 17% |
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Unknown | 11 | 17% |