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Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii

Overview of attention for article published in Plant Journal, March 2009
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (91st percentile)
  • High Attention Score compared to outputs of the same age and source (96th percentile)

Mentioned by

patent
5 patents
peer_reviews
1 peer review site
wikipedia
1 Wikipedia page

Citations

dimensions_citation
315 Dimensions

Readers on

mendeley
410 Mendeley
connotea
1 Connotea
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Title
Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii
Published in
Plant Journal, March 2009
DOI 10.1111/j.1365-313x.2008.03767.x
Pubmed ID
Authors

Attila Molnar, Andrew Bassett, Eva Thuenemann, Frank Schwach, Shantanu Karkare, Stephan Ossowski, Detlef Weigel, David Baulcombe

Abstract

MicroRNAs (miRNAs) are small RNAs, 21 to 22 nucleotides long, with important regulatory roles. They are processed from longer RNA molecules with imperfectly matched foldback regions and they function in modulating the stability and translation of mRNA. Recently, we and others have demonstrated that the unicellular alga Chlamydomonas reinhardtii, like diverse multicellular organisms, contains miRNAs. These RNAs resemble the miRNAs of land plants in that they direct site-specific cleavage of target mRNA with miRNA-complementary motifs and, presumably, act as regulatory molecules in growth and development. Utilizing these findings we have developed a novel artificial miRNA system based on ligation of DNA oligonucleotides that can be used for specific high-throughput gene silencing in green algae.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 410 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 9 2%
Germany 2 <1%
Netherlands 2 <1%
Chile 1 <1%
France 1 <1%
Kenya 1 <1%
Colombia 1 <1%
Brazil 1 <1%
Taiwan 1 <1%
Other 6 1%
Unknown 385 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 121 30%
Researcher 88 21%
Student > Master 43 10%
Student > Bachelor 42 10%
Professor > Associate Professor 19 5%
Other 59 14%
Unknown 38 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 266 65%
Biochemistry, Genetics and Molecular Biology 76 19%
Environmental Science 8 2%
Engineering 6 1%
Chemistry 2 <1%
Other 7 2%
Unknown 45 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 October 2023.
All research outputs
#2,655,923
of 25,374,917 outputs
Outputs from Plant Journal
#643
of 7,298 outputs
Outputs of similar age
#8,372
of 106,836 outputs
Outputs of similar age from Plant Journal
#1
of 27 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 7,298 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.5. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 106,836 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.