| Title |
Nutritional and metabolic stressors on ovine oocyte development and granulosa cell functions in vitro
|
|---|---|
| Published in |
Cell Stress and Chaperones, October 2017
|
| DOI | 10.1007/s12192-017-0846-1 |
| Pubmed ID | |
| Authors |
S. Nandi, S.K. Tripathi, P.S.P. Gupta, S. Mondal |
| Abstract |
The present study was undertaken to study the effect of ammonia, urea, non-esterified fatty acid (NEFA), and β-hydroxybutyric acid (β-OHB) on oocyte development and granulosa cell (GC) growth parameter of ovine (Ovis aries). Ovine oocytes were matured in vitro in the presence of different concentration of ammonia, urea, NEFA, and β-OHB for 24 h, in vitro inseminated and evaluated for cleavage and blastocyst yield. Same concentrations of ammonia, urea, NEFA, and β-OHB were examined on growth parameters and hormone secretion activity of granulosa cells in vitro. Real-time reverse transcription polymerase chain reaction was used to evaluate the expression of steroidogenic genes (steroidogenic cytochrome P-450 (CYP11A1, CYP19A1)), cell proliferation-related genes (GDF9, FSHr), and apoptosis-related genes (BCL-2 and BAX). The maturation, cleavage, and blastocyst production rates were significantly lowered in media containing either 200 μM ammonia or 5 mM urea or high combo NEFA or 1 μM β-OHB. Exposure of granulosa cell to 400 μM ammonia or 1 μM β-OHB or very high combo or 6 mM urea significantly decreased all the parameters examined compared to lower levels of all nutritional and metabolic stressors. Elevated concentration of metabolic stressors induced GC apoptosis through the BAX/BCL-2 pathway and reduced the steroidogenic gene messenger RNA (mRNA) expression and cell proliferation gene mRNA expression. These results suggested that the decreased function of GCs may cause ovarian dysfunction and offered an improved understanding of the molecular mechanism responsible for the low fertility in metabolic stressed condition. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 25 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 12% |
| Researcher | 3 | 12% |
| Student > Doctoral Student | 2 | 8% |
| Other | 2 | 8% |
| Student > Bachelor | 2 | 8% |
| Other | 3 | 12% |
| Unknown | 10 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 32% |
| Biochemistry, Genetics and Molecular Biology | 2 | 8% |
| Chemistry | 2 | 8% |
| Medicine and Dentistry | 1 | 4% |
| Arts and Humanities | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 11 | 44% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 October 2017.
All research outputs
#17,302,400
of 25,394,764 outputs
Outputs from Cell Stress and Chaperones
#417
of 699 outputs
Outputs of similar age
#212,329
of 332,238 outputs
Outputs of similar age from Cell Stress and Chaperones
#6
of 14 outputs
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So far Altmetric has tracked 699 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 32nd percentile – i.e., 32% of its peers scored the same or lower than it.
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