| Title |
Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase
|
|---|---|
| Published in |
Microbial Cell Factories, October 2017
|
| DOI | 10.1186/s12934-017-0783-9 |
| Pubmed ID | |
| Authors |
Liang Tian, Skyler J. Perot, Shuen Hon, Jilai Zhou, Xiaoyu Liang, Jason T. Bouvier, Adam M. Guss, Daniel G. Olson, Lee R. Lynd |
| Abstract |
Pyruvate decarboxylase (PDC) is a well-known pathway for ethanol production, but has not been demonstrated for high titer ethanol production at temperatures above 50 °C. Here we examined the thermostability of eight PDCs. The purified bacterial enzymes retained 20% of activity after incubation for 30 min at 55 °C. Expression of these PDC genes, except the one from Zymomonas mobilis, improved ethanol production by Clostridium thermocellum. Ethanol production was further improved by expression of the heterologous alcohol dehydrogenase gene adhA from Thermoanaerobacterium saccharolyticum. The best PDC enzyme was from Acetobactor pasteurianus. A strain of C. thermocellum expressing the pdc gene from A. pasteurianus and the adhA gene from T. saccharolyticum was able to produce 21.3 g/L ethanol from 60 g/L cellulose, which is 70% of the theoretical maximum yield. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 74 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 22% |
| Student > Master | 11 | 15% |
| Researcher | 10 | 14% |
| Student > Bachelor | 8 | 11% |
| Student > Doctoral Student | 6 | 8% |
| Other | 7 | 9% |
| Unknown | 16 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 24 | 32% |
| Agricultural and Biological Sciences | 11 | 15% |
| Engineering | 6 | 8% |
| Chemical Engineering | 5 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 3% |
| Other | 5 | 7% |
| Unknown | 21 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 October 2017.
All research outputs
#14,303,442
of 23,005,189 outputs
Outputs from Microbial Cell Factories
#876
of 1,612 outputs
Outputs of similar age
#177,642
of 323,110 outputs
Outputs of similar age from Microbial Cell Factories
#12
of 38 outputs
Altmetric has tracked 23,005,189 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,612 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 323,110 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 38 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.