| Title |
Characterisation of human induced pluripotent stem cell-derived endothelial cells under shear stress using an easy-to-use microfluidic cell culture system
|
|---|---|
| Published in |
Biomedical Microdevices, October 2017
|
| DOI | 10.1007/s10544-017-0229-5 |
| Pubmed ID | |
| Authors |
Rsituko Ohtani-Kaneko, Kenjiro Sato, Atsuhiro Tsutiya, Yuka Nakagawa, Kazutoshi Hashizume, Hidekatsu Tazawa |
| Abstract |
Induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) can contribute to elucidating the pathogenesis of heart and vascular diseases and developing their treatments. Their precise characteristics in fluid flow however remain unclear. Therefore, the aim of the present study is to characterise these features. We cultured three types of ECs in a microfluidic culture system: commercially available human iPS-ECs, human umbilical vein endothelial cells (HUVECs) and human umbilical artery endothelial cells (HUAECs). We then examined the mRNA expression levels of endothelial marker gene cluster of differentiation 31 (CD31), fit-related receptor tyrosine kinase (Flk-1), and the smooth muscle marker gene smooth muscle alpha-actin, and investigated changes in plasminogen activator inhibitor-1 (PAI-1) secretion and intracellular F-actin arrangement following heat stress. We also compared expressions of the arterial and venous marker genes ephrinB2 and EphB4, and the endothelial gap junction genes connexin (Cx) 37, 40, and 43 under fluidic shear stress to determine their arterial or venous characteristics. We found that iPS-ECs had similar endothelial marker gene expressions and exhibited similar increases in PAI-1 secretion under heat stress as HUVECs and HUAECs. In addition, F-actin arrangement in iPSC-ECs also responded to heat stress, as previously reported. However, they had different expression patterns of arterial and venous marker genes and Cx genes under different fluidic shear stress levels, showing that iPSC-ECs exhibit different characteristics from arterial and venous ECs. This microfluidic culture system equipped with variable shear stress control will provide an easy-to-use assay tool to examine characteristics of iPS-ECs generated by different protocols in various laboratories and contribute to basic and applied biomedical researches on iPS-ECs. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 52 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 19% |
| Researcher | 10 | 19% |
| Student > Master | 9 | 17% |
| Student > Bachelor | 4 | 8% |
| Student > Postgraduate | 3 | 6% |
| Other | 6 | 12% |
| Unknown | 10 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 12 | 23% |
| Biochemistry, Genetics and Molecular Biology | 9 | 17% |
| Agricultural and Biological Sciences | 5 | 10% |
| Medicine and Dentistry | 5 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
| Other | 8 | 15% |
| Unknown | 11 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 October 2017.
All research outputs
#20,449,496
of 23,005,189 outputs
Outputs from Biomedical Microdevices
#672
of 754 outputs
Outputs of similar age
#283,085
of 324,598 outputs
Outputs of similar age from Biomedical Microdevices
#5
of 9 outputs
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