Chapter title |
Chromosome Painting by GISH and Multicolor FISH
|
---|---|
Chapter number | 2 |
Book title |
Plant Cytogenetics
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3622-9_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3620-5, 978-1-4939-3622-9
|
Authors |
Steven S. Xu, Zhao Liu, Qijun Zhang, Zhixia Niu, Chao-Chien Jan, Xiwen Cai, Xu, Steven S., Liu, Zhao, Zhang, Qijun, Niu, Zhixia, Jan, Chao-Chien, Cai, Xiwen |
Abstract |
Fluorescent in situ hybridization (FISH) is a powerful cytogenetic technique for identifying chromosomes and mapping specific genes and DNA sequences on individual chromosomes. Genomic in situ hybridization (GISH) and multicolor FISH (mc-FISH) represent two special types of FISH techniques. Both GISH and mc-FISH experiments have general steps and features of FISH, including chromosome preparation, probe labeling, blocking DNA preparation, target-probe DNA hybridization, post-hybridization washes, and hybridization signal detection. Specifically, GISH uses total genomic DNA from two species as probe and blocking DNA, respectively, and it can differentiate chromosomes from different genomes. The mc-FISH takes advantage of simultaneous hybridization of several DNA probes labeled by different fluorochromes to different targets on the same chromosome sample. Hybridization signals from different probes are detected using different fluorescence filter sets. Multicolor FISH can provide more structural details for target chromosomes than single-color FISH. In this chapter, we present the general experimental procedures for these two techniques with specific details in the critical steps we have modified in our laboratories. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 5 | 24% |
Student > Ph. D. Student | 3 | 14% |
Student > Master | 2 | 10% |
Researcher | 2 | 10% |
Student > Doctoral Student | 1 | 5% |
Other | 2 | 10% |
Unknown | 6 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 29% |
Agricultural and Biological Sciences | 5 | 24% |
Unspecified | 1 | 5% |
Medicine and Dentistry | 1 | 5% |
Neuroscience | 1 | 5% |
Other | 1 | 5% |
Unknown | 6 | 29% |