Title |
DNA Ligase-Based Strategy for Quantifying Heterogeneous DNA Methylation without Sequencing
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Published in |
Clinical Chemistry, January 2015
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DOI | 10.1373/clinchem.2014.227546 |
Pubmed ID | |
Authors |
Eugene J H Wee, Sakandar Rauf, Muhammad J A Shiddiky, Alexander Dobrovic, Matt Trau |
Abstract |
DNA methylation is a potential source of disease biomarkers. Typically, methylation levels are measured at individual cytosine/guanine (CpG) sites or over a short region of interest. However, regions of interest often show heterogeneous methylation comprising multiple patterns of methylation (epialleles) on individual DNA strands. Heterogeneous methylation is largely ignored because digital methods are required to deconvolute these usually complex patterns of epialleles. Currently, only single-molecule approaches, such as next generation sequencing (NGS), can provide detailed epiallele information. Because NGS is not yet feasible for routine practice, we developed a single-molecule-like approach, named for epiallele quantification (EpiQ). |
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France | 1 | 33% |
India | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
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Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
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Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 16% |
Student > Bachelor | 2 | 11% |
Other | 2 | 11% |
Student > Master | 2 | 11% |
Other | 1 | 5% |
Unknown | 5 | 26% |
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Environmental Science | 1 | 5% |
Other | 0 | 0% |
Unknown | 6 | 32% |