| Title |
Molecular characterization of carbapenem-resistant Enterobacteriaceae at a tertiary care laboratory in Mumbai
|
|---|---|
| Published in |
European Journal of Clinical Microbiology & Infectious Diseases, September 2014
|
| DOI | 10.1007/s10096-014-2249-x |
| Pubmed ID | |
| Authors |
M. Kazi, L. Drego, C. Nikam, K. Ajbani, R. Soman, A. Shetty, C. Rodrigues |
| Abstract |
Carbapenem-hydrolyzing β-lactamases are increasingly reported worldwide, leading to therapeutic failure. In an era where the drug development pipeline is stagnant, it is crucial to preserve current classes of antibiotics to help fight against infection caused by multidrug-resistant organisms (MDROs), by practicing a rational approach for the use of antibiotics. Identifying the mechanisms of resistance gives us much needed insights in this field. A total of 113 consecutive, non-duplicate carbapenem-resistant clinical isolates were collected from July to December 2012. These isolates were subjected to the modified Hodge test (MHT) for phenotypic detection of carbapenemases, an inhibitor-based test employing EDTA for the detection of metallo-β-lactamase (MBL), and phenylboronic acid for the detection of Klebsiella pneumoniae carbapenemase (KPC). A multiplex polymerase chain reaction (PCR) assay that characterized the five most predominant carbapenemases (bla NDM, bla OXA, bla VIM, bla IMP, bla KPC) was designed. The 113 isolates consisted of Klebsiella spp. (46), Enterobacter spp. (32), Escherichia coli (31), Citrobacter spp. (2), Proteus spp. (1), and Morganella spp. (1). bla NDM-1 was the most prevalent carbapenemase and accounted for 75.22 % (85/113) of the isolates. This was followed by bla OXA [4.42 % (n = 5)]. 18.5 % (21/113) of the isolates possessed dual carbapenemase genes. 98.9 % concordance was observed between the phenotypic tests and the molecular tests for the detection of MBL. In conclusion, patients infected with resistant bacteria require early appropriate antimicrobial treatment for good clinical outcome. Thus, identifying the resistant mechanisms of suspected pathogens becomes crucial. Also, the high incidence of plasmid-mediated bla NDM-1 calls for the implementation of strict infection control and contact isolation precautions in order to prevent the spread of these organisms. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 33% |
| Spain | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 86 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 86 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 19% |
| Student > Postgraduate | 11 | 13% |
| Researcher | 10 | 12% |
| Student > Master | 7 | 8% |
| Student > Bachelor | 6 | 7% |
| Other | 14 | 16% |
| Unknown | 22 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 25 | 29% |
| Immunology and Microbiology | 10 | 12% |
| Biochemistry, Genetics and Molecular Biology | 8 | 9% |
| Agricultural and Biological Sciences | 4 | 5% |
| Environmental Science | 3 | 3% |
| Other | 7 | 8% |
| Unknown | 29 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 November 2015.
All research outputs
#14,786,597
of 22,765,347 outputs
Outputs from European Journal of Clinical Microbiology & Infectious Diseases
#1,779
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Outputs of similar age
#139,247
of 252,541 outputs
Outputs of similar age from European Journal of Clinical Microbiology & Infectious Diseases
#11
of 23 outputs
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